Ectomycorrhiza-specific gene expression: Function and regulation

外生菌根特异性基因表达：功能和调控

• 批准号: 5399425
• 负责人: Professor Dr. Uwe Nehls
• 金额: --
• 依托单位: Arbeitsgruppe Botanik
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2003
• 资助国家: 德国
• 起止时间: 2002-12-31 至 2008-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5399425?language=en
• 关键词: Ectomycorrhiza; specific; gene; expression; Function

Poplar could succeed in nutrient rich areas as well as in nutrient poor forests soils where plants live in symbiosis with certain soil fungi to enable sufficient nutrition. Due to its huge demand, nitrogen, as major nutrient, is of special interest for poplar nutrition. In this project we want to characterize nitrate, ammonium and amino acid transporters from poplar roots that are differentially regulated as result of nitrogen nutrition (shortage or nitrogen excess), or by plant/fungus interaction. The kinetic parameters of selected transporters will be determined by heterologous expression. Tissue and organ specific expression of certain transporter genes will be investigated by Northern blot and RT-PCR and by the utilization of poplar transformants containing promoter-GFP fusions. GFP fusions with truncated promoters will also be used for the identification of cis-elements responsible for the nitrogen-dependent expression of selected transporter genes. In addition, the global impact of nitrogen nutrition on poplar gene expression will be investigated using macro and micro arrays hybridization and probes of poplar roots grown at different nitrogen sources and concentrations as well as mycorrhizas.

杨树既可以在养分丰富的地区生长，也可以在养分贫乏的森林土壤中生长，在这些土壤中植物与某些土壤真菌共生以获得足够的营养。由于需要量巨大，氮作为杨树的主要营养物质，是杨树营养研究的重点。在这个项目中，我们想要表征杨树根系中的硝酸盐、铵和氨基酸转运体，这些转运体受氮营养（氮短缺或氮过剩）或植物/真菌相互作用的差异调节。所选转运体的动力学参数将通过异源表达确定。某些转运基因的组织和器官特异性表达将通过Northern blot和RT-PCR以及利用含有启动子- gfp融合的杨树转化体进行研究。与截断启动子的GFP融合也将用于鉴定负责选定转运体基因的氮依赖表达的顺式元件。此外，本研究还将利用宏、微阵列杂交技术和探针对不同氮源、不同氮浓度的杨树根系及菌根进行研究，探讨氮营养对杨树基因表达的影响。