vascular invasion and cultivated oral mucosal epithelial sheets

血管侵犯和培养的口腔粘膜上皮片

• 批准号: 22591951
• 负责人: SATAKE Yoshiyuki
• 金额: $ 2.83万
• 依托单位: Tokyo Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22591951/
• 关键词: 移植・再生医療; 眼表面再建; 血管新生

Transplantation of cultivated oral mucosal epithelial sheets is one of the surgical procedures to reconstruct ocular surface in patients with limbal stem cell deficiency. The major advantage of this treatment is the absence of the need for postoperative immunosuppressive therapy. There are two types of cultivated oral mucosal epithelial sheets with or without amniotic membrane as a substrate. Since vascular invasion underneath transplanted sheet, which might cause decreased visual acuities were common in cases of the transplantation of cultivated oral mucosal epithelium sheets with a substrate during postoperative courses, this finding was considered a possibility of the existence substrate is involved in the pathogenesis. First, I examined the relationship between the existence of substrate and the expression of angiogeneic growth factors and angiogenesis inhibitors in cultivated oral mucosal epithelial sheets. There was no difference of the expression of these factors at both transcr … More iption and protein level. Next, the histopathological examination of corneal tissue harvested at the time of secondary keratoplasty following the transplantation of cultivated oral mucosal epithelial transplantation, and examined was performed. Each pathological finding had strong influence of microenvironment of ocular surface, and it was in the difficult situation to confirm the influence by the existence of a substrate. Therefore, we examined histopathologically the prognosis of cultivated epithelial sheets prepared from rabbitoral mucosa by using the rabbit model of limbus stem cell dysfunction. Cultivated epithelial sheets without substrate obtained good adhesion to corneal stroma and smooth ocular surface. On the other hand, cultivated epithelial sheets with substrate had low adhesion and epithelial cell invasion and vascular invasion accompanied with fibrosis from the peripheral underneath transplanted epithelial sheets were observed. Similar findings of vascular invasion underneath transplanted oral mucosal epithelial sheets also obtained in rabbit model. These data suggests the adhesiveness with transplanted oral mucosal epithelial sheets and corneal stroma may cause the vascular invasion underneath transplanted sheets. Less

培养的口腔黏膜上皮片移植是重建角膜缘干细胞缺乏症患者眼表的手术方法之一。这种治疗的主要优点是不需要术后免疫抑制治疗。有两种类型的培养口腔黏膜上皮片，有羊膜或没有羊膜作为基质。由于种植口腔黏膜上皮片移植过程中，移植片下血管的侵袭是常见的，可能导致视力下降，这一发现被认为是存在底物参与发病机制的可能性。首先，我研究了培养的口腔黏膜上皮片中底物的存在与血管生成生长因子和血管生成抑制因子表达的关系。在两种Transcr…中，这些因子的表达没有差异更多的脂肪和蛋白质水平。接下来，对培养的口腔黏膜上皮移植后二次角膜移植时采集的角膜组织进行组织病理学检查，并进行检查。每一种病理发现都受到眼表微环境的强烈影响，而底物的存在对其影响难以确定。因此，我们采用兔角膜缘干细胞功能障碍模型，对兔眼黏膜上皮细胞培养后的预后进行了组织病理学研究。无基质培养上皮片与角膜基质粘附性好，眼表光滑。另一方面，有基质的培养上皮片粘附率低，移植上皮片下可见上皮细胞侵袭和血管侵袭并伴有纤维化。在兔模型中，移植的口腔黏膜上皮片下也有类似的血管侵袭发现。这些数据提示口腔黏膜上皮片与角膜基质的粘附性可能导致移植片下血管的侵袭。较少

项目成果

角膜輪部機能不全に対する培養上皮シート移植後の二期的角膜移植の成績

培养上皮片移植后二期角膜移植治疗角膜缘功能障碍的结果

• 发表时间: 2010
• 作者: Ohguro H;Ohguro I;Katai M;Tanaka S;佐竹良之
• 通讯作者: 佐竹良之