Glycolate oxidation inside the chloroplast: A novel method for the suppression of photorespiration in plants

叶绿体内的乙醇酸氧化：抑制植物光呼吸的新方法

• 批准号: 5399568
• 负责人: Professor Dr. Christoph Peterhänsel
• 金额: --
• 依托单位: Institut für Botanik
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2003
• 资助国家: 德国
• 起止时间: 2002-12-31 至 2006-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5399568?language=en
• 关键词: Glycolate; oxidation; inside; chloroplast; novel

Plants loose a significant part of the already fixed CO2 in the process of photorespiration. This effect is exacerbated further under hot and arid climates. Also, growth and yield of crops is clearly enhanced by fertilisation with CO2 under otherwise optimal conditions. We propose a novel approach for the suppression of photorespiration. A pathway derived from E. coli will be transferred into the chloroplast capable of converting glycolate into glycerate under release of CO2. Thus, the products of photorespiration will directly lead to enhanced CO2 concentrations in the chloroplast and therefore suppress photorespiration. Important novelties of this approach are the identification and application of glycolate-oxidising enzymes with organic co-factors avoiding the production of oxygen radicals and the transfer of the complete coding sequences as one cistron into the chloroplast genome.

植物在光呼吸过程中释放出相当大一部分已经固定的CO2。这种影响在炎热和干旱的气候下进一步加剧。此外，在其他最佳条件下，CO2施肥明显提高了作物的生长和产量。我们提出了一种新的方法来抑制光呼吸。从E.大肠杆菌将被转移到能够在CO2释放下将乙醇酸转化为甘油酸的叶绿体中。因此，光呼吸的产物将直接导致叶绿体中CO2浓度的增加，从而抑制光呼吸。这种方法的重要新颖之处是乙醇酸氧化酶与有机辅因子的鉴定和应用，避免了氧自由基的产生，并将完整的编码序列作为一个顺反子转移到叶绿体基因组中。