Maintenance mechanism of oral homeostasis revealed by fine scale analysis of microbiota

口腔暴露在许多复杂的物质中，是细菌侵入人体的第一扇门。本研究的目的是精确地进行口腔植物群分析，并旨在阐明口腔微生物环境如何保持恒定的机制。口腔环境变化复杂，目前尚无相关资料。因此，本研究对正常口腔植物群、龋病和牙周病进行了细菌群落的比较分析，旨在通过对维持口腔内环境稳定的关键细菌的分离和基因组测定，阐明口腔内环境稳定的维持机制。结果，在10岁左右的儿童中，细菌与成人没有什么不同。另一方面，作为分离菌株的基因组分析的结果，微生态位中的细菌存活可能受到CRISPR的极大影响，CRISPR是一种细菌获得性免疫机制。这些知识成为未来宏基因组分析的基础。

The oral cavity is exposed to many complex substances and is the first door for bacteria to invade the body. The goal of this study is to perform the oral flora analysis precisely and is intended to clarify the mechanism that how constancy of the oral microbial environment is maintained. The oral environment changes complicatedly, and there is no data about Archaea. Therefore comparative bacterial community analysis was carried out using normal oral flora, caries and periodontal disease, aimed at the elucidation of the oral constancy maintenance mechanism through isolation and genome determination of the key bacteria to the homeostasis. As a result, in the children of around 10 years old, the bacteria were not different with those of an adult. On the other hand, as a result of the genome analysis of the isolated strains, bacterial survival in the niche could be affected greatly by CRISPR which was a bacterial acquired immunity mechanism. The knowledge becomes the base of future metagenome analysis.