Maintenance mechanism of oral homeostasis revealed by fine scale analysis of microbiota

微生物群精细分析揭示口腔稳态的维持机制

• 批准号: 22592032
• 负责人: MARUYAMA Fumito
• 金额: $ 2.83万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22592032/
• 关键词: 細菌叢; 新型シーケンサー; 恒常性; 口腔細菌叢; 細菌ゲノム; 高速シーケンサー

The oral cavity is exposed to many complex substances and is the first door for bacteria to invade the body. The goal of this study is to perform the oral flora analysis precisely and is intended to clarify the mechanism that how constancy of the oral microbial environment is maintained. The oral environment changes complicatedly, and there is no data about Archaea. Therefore comparative bacterial community analysis was carried out using normal oral flora, caries and periodontal disease, aimed at the elucidation of the oral constancy maintenance mechanism through isolation and genome determination of the key bacteria to the homeostasis. As a result, in the children of around 10 years old, the bacteria were not different with those of an adult. On the other hand, as a result of the genome analysis of the isolated strains, bacterial survival in the niche could be affected greatly by CRISPR which was a bacterial acquired immunity mechanism. The knowledge becomes the base of future metagenome analysis.

口腔暴露在许多复杂的物质中，是细菌侵入人体的第一扇门。本研究的目的是精确地进行口腔植物群分析，并旨在阐明口腔微生物环境如何保持恒定的机制。口腔环境变化复杂，目前尚无相关资料。因此，本研究对正常口腔植物群、龋病和牙周病进行了细菌群落的比较分析，旨在通过对维持口腔内环境稳定的关键细菌的分离和基因组测定，阐明口腔内环境稳定的维持机制。结果，在10岁左右的儿童中，细菌与成人没有什么不同。另一方面，作为分离菌株的基因组分析的结果，微生态位中的细菌存活可能受到CRISPR的极大影响，CRISPR是一种细菌获得性免疫机制。这些知识成为未来宏基因组分析的基础。

项目成果

Complete Genome Sequence of the Bacterium Porphyromonas gingivalis TDC60, Which Causes Periodontal Disease

• DOI: 10.1128/jb.05269-11
• 发表时间: 2011-08-01
• 期刊: JOURNAL OF BACTERIOLOGY
• 影响因子: 3.2
• 作者: Watanabe, Takayasu;Maruyama, Fumito;Abiko, Yoshimitsu
• 通讯作者: Abiko, Yoshimitsu

Transition metal ions induce carnosinase activity in PepD-homologous protein from Porphyromonas gingivalis TDC60 with type II fimA

过渡金属离子通过 II 型 fimA 诱导牙龈卟啉单胞菌 TDC60 的 PepD 同源蛋白中的肌肽酶活性

• 发表时间: 2012
• 期刊: Microb. Pathog
• 作者: A. Aoki;Y. Shibata;S. Okano;F.Maruyama;A. Amano;I. Nakagawa,*Y. Abiko
• 通讯作者: I. Nakagawa,*Y. Abiko

Species-specific strategy of genome diversification in genus streptococcivia prophage acquisition and its defense system

链球菌属原噬菌体获取及其防御系统基因组多样化的物种特异性策略

• 发表时间: 2011
• 作者: A. Sakurai;F. Maruyama;J. Funao;T.Nozawa;C. Aikawa;N. Okahashi;S. Shintani;S. Hamada;*T. Ooshima;I.Nakagawa;Aikawa Chihiro;Mori Hiroshi;Takahashi Ichiro;Sakurai Atsuo;Aikawa Chihiro;Maruyama Fumito
• 通讯作者: Maruyama Fumito

東京医科歯科大学・大学院医歯学総合研究科・環境遺伝生態学分野

东京医科齿科大学医学齿科研究生院环境遗传生态学系

visualization tool for taxonomic compositions of microbial communities based on 16S rRNA gene sequences

基于16S rRNA基因序列的微生物群落分类组成可视化工具

• 发表时间: 2010
• 期刊: BMC Biofinfo
• 作者: H. Mori;F. Maruyama;K. Kurokawa.VITCOMIC
• 通讯作者: K. Kurokawa.VITCOMIC