Study on the Glu-specific protease superfamily mediating dissociation of cell-cell adhesion

谷氨酸特异性蛋白酶超家族介导细胞间粘附解离的研究

• 批准号: 22592070
• 负责人: NEMOTO Yuko
• 金额: $ 2.83万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22592070/
• 关键词: セリンプロテアーゼ; プロテアーゼ; リコンビナントタンパク質; 病原因子; 黄色ブドウ球菌; 活性化; ジペプチジルペプチダーゼ; ブドウ球菌; 酵素

Shortening of the propeptide of Glu-specific serine peptidase(GluV8) family commonly occurred in an auto-catalytic manner. Accompanied with propeptide-shortening, the pro-mature junction became more susceptible toward the maturation enzymes. We demonstrated the in vitro Glu-specific endopeptidase activity of exfoliative toxin (ET) A for the first time, which was measured with Escherichia coli-expressed recombinant ETAs and synthetic peptidyl substrates. The animal model revealed that the proteolytic activity was indispensable for exfoliation of the skin. In addition, we determined characteristics of DPP11 and DPP7, and proposed a novel phylogenetic system of these family molecules.

谷氨酸特异性丝氨酸肽酶（GluV8）家族的前肽的缩短通常以自催化方式发生。伴随着前肽缩短，前成熟连接变得更容易受到成熟酶的影响。我们证明了在体外Glu特异性内肽酶活性的剥脱毒素（ET）A的第一次，这是大肠杆菌表达的重组ETA和合成肽基底物测定。动物模型显示，蛋白水解活性是皮肤脱落所不可缺少的。此外，我们确定了DPP11和DPP7的特征，并提出了一个新的系统发育的这些家庭分子。

项目成果

Relationship between oral status and prevalence of periodontopathic bacteria on tongue of 85-years elderly.

85岁老年人口腔状况与舌部牙周病菌患病率的关系

• 发表时间: 2010
• 期刊: Journal of Medical Microbiology
• 影响因子: 3
• 作者: Eiko Sakai;Megumi Sugawara;Kazuhisa Nishishita;Yutaka Fukuma;Kuniaki Okamoto and Takayuki Tsukuba;Ono Toshio;Kishi Mitsuo
• 通讯作者: Kishi Mitsuo

A novel Asp and Glu specific dipeptitidyl peptidase from Porphyromonas endodontalis

一种来自牙髓卟啉单胞菌的新型天冬氨酸和谷氨酸特异性二肽基肽酶

• 发表时间: 2011
• 作者: 根津顕弘;谷村明彦;森田貴雄;東城庸介;Tetuo Fumi
• 通讯作者: Tetuo Fumi

Auto-catalytic processing of prosequence prior to maturation of Staphylococcal glutamyl endopeptidases.

葡萄球菌谷氨酰内肽酶成熟前原序列的自动催化加工。

• 发表时间: 2010
• 作者: Tabata K;Sakai H;Nakajima R;Saya-Nishimura R;Motani K;Okano S;Shibata Y;Abiko Y;Suzuki T;Shakh M.A.Rouf
• 通讯作者: Shakh M.A.Rouf

Expression and Characterization of Dipeptidyl-Peptidases of Porphyromonas gingivalis

牙龈卟啉单胞菌二肽基肽酶的表达和表征

• 发表时间: 2012
• 作者: Rouf;Shakh M. A.;Ohara-Nemoto;Y.;Yanase;A.;Ono T.;and Nemoto;T. K.
• 通讯作者: T. K.

表皮剥奪毒素ETA N 末端領域の生物活性に及ぼす作用

表皮脱叶毒素ETA对N端区域生物活性的影响

• 发表时间: 2011
• 作者: 村田佳織;森田貴雄;根津顕弘;齊藤正人;谷村明彦;達聖月,根本優子,馬場友巳,小早川健,藤田修一,池田通,大井久美子,根本孝幸
• 通讯作者: 達聖月,根本優子,馬場友巳,小早川健,藤田修一,池田通,大井久美子,根本孝幸