Sensitization of trigeminal nervous system by infraorbital nerve injury exacerbates the pathophysiology of migraine

采用离子松弛结扎法建立大鼠眶下神经慢性压迫性损伤模型(ION-CCI)，建立慢性口面部疼痛模型(神经病理性疼痛模型)。在离子-CCI后3周，用10 mM辣椒素刺激硬脑膜，用免疫组织化学方法检测三叉神经脊束核尾侧亚核磷酸化细胞外信号调节激酶(PERK)的表达。结果表明，与辣椒素刺激组相比，离子-CCl+辣椒素刺激组大鼠卵巢至1440μm的PERK免疫反应阳性神经元在尾侧明显增多。综上所述，离子-CCI对三叉神经第二支的敏化影响了辣椒素对三叉神经第一支的激活作用，提示慢性口面部疼痛可能加重了偏头痛的病理生理过程。

We established the chronic constriction injury of the infraorbital nerve (ION-CCI) rat model as a chronic orofacial pain model (neuropathic pain model) by ION loose ligation. At 3 weeks after ION-CCI, the dura was stimulated by 10mM capsaicin, which was the one of the migraine models, followed by checking the expression of the phosphorylated extracellular signal-regulated kinase (pERK) at the trigeminal spinal subnucleus caudalis by immunohistochemical staining method. It showed that the pERK-immunoreactive neurons statistically increased between ovex and 1440 μm caudally in ION-CCI + capisaisin stimulating group compared to capsaicin stimulating group. Taken together, the sensitization of 2nd branch of trigeminal nerve by ION-CCI affected the activation of 1stbranch of trigeminal nerve by capsaicin, suggesting that chronic orofacial pain may exacerbates the pathophysiology of migraine.