Comprehensive approaches for investigating the neural network dynamics in C. elegans

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We have developed a new technique for investigating the neural circuit and neurons comprehensively with micro-fluid devices and fluorescent proteins. Our main outcomes and results are followings.We developed specific micro-fluid devices for controlling the microenvironment around the worm precisely, and also developed a software for analyzing the behavior of the head region of the worm.We succeeded to visualize the neural activity with fluorescent-based Ca sensor and also membrane potential sensor during odorant stimulation in the micro-fluidic devices. We also succeeded to selectively eliminate the specific neurons with Killerred, that produces reactive oxygen species(ROS) during green light illumination.The combination of these techniques enable us to investigate the relationships between the neuronal activities and behaviors in detail and comprehensively.