Development of genetic manipulation technology for excitatory and inhibitory control of the target neuron activity

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In the present study, we aimed to develop a novel technology for excitatory and inhibitory control of the activity of target neuronal types. A single chain variable region of anti-Tac monoclonal antibody against human interleukin-2 receptor a-subunit was fused to Fc fragment[anti-Tac(Fv)-Fc] was bound to liposome to produce immunoliposome. Anti-Tac(Fv) was connected to the transmembrane domain of vesicular somatitis virus glycoprotein or VSV-G[anti-Tac(Fv)-VSV-G], and anti-Tac(Fv)-VSV-G was expressed in insect cells by the baculoviral vector system. These cells were fused to loposome to generate proteoliposome containing anti-Tac(Fv)-VSV-G. We succeeded in the reduction of nonspecific binding of immunoliposome and proteoliposome by adding unlabelled control liposome into the solution containing the rhodamine-labelled liposome. This strategy will be useful to control the activity of neurons by introducing some drugs that inhibit ion channels into immunoliposome or proteoliposome.