Analysis of molecular dynamics in neuronal dendrites by simultaneous multi-point fluorescence correlation spectroscopy

通过同时多点荧光相关光谱分析神经元树突的分子动力学

• 批准号: 22650066
• 负责人: INOUE Takafumi
• 金额: $ 2.24万
• 依托单位: Waseda University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22650066/
• 关键词: 分子; 細胞神経科学; 蛍光相関分光法; タンパク質; 拡散; 樹状突起; スパイン; 2光子励起顕微鏡; 分子・細胞神経科学

This research project aimed to measure dynamics of protein molecules rapidly moving in cytosole of neuronal dendrites at multiple points simultaneously by means of a newly developed two-photon microscope. I succeeded in recording at multiple points at more than 10 kHz time of resolution from cytosol of primary cultured neurons derived from mouse, in which GFP or GFP-fused CamKII protein was expressed, and in analyzing by FCS.

本研究旨在利用双光子显微镜对神经元树突胞浆中蛋白质分子的多点快速运动动力学进行测量。本人成功地从表达GFP或GFP融合的CamKII蛋白的小鼠原代培养神经元的胞质溶胶中以超过10 kHz的时间在多个点记录分辨率，并通过FCS进行分析。

项目成果

ランダムスキャン2光子励起顕微鏡による多点同時FCSの試み

尝试使用随机扫描双光子激发显微镜进行多点同时 FCS

• 发表时间: 2011
• 作者: S.Morteza Heidarinejad;井上 貴文
• 通讯作者: 井上 貴文

Hyperforin modulates dendritic spine morphology in hippocampal pyramidal neurons by activating Ca(2+) -permeable TRPC6 channels.

• DOI: 10.1002/hipo.22052
• 发表时间: 2013-01
• 期刊: HIPPOCAMPUS
• 影响因子: 3.5
• 作者: Leuner, Kristina;Li, Wei;Amaral, Michelle D.;Rudolph, Stephanie;Calfa, Gaston;Schuwald, Anita M.;Harteneck, Christian;Inoue, Takafumi;Pozzo-Miller, Lucas
• 通讯作者: Pozzo-Miller, Lucas

Chemically inducible diffusion trap at cilia reveals molecular sieve-like barrier.

• DOI: 10.1038/nchembio.1252
• 发表时间: 2013-07
• 期刊: NATURE CHEMICAL BIOLOGY
• 影响因子: 14.8
• 作者: Lin, Yu-Chun;Niewiadomski, Pawel;Lin, Benjamin;Nakamura, Hideki;Phua, Siew Cheng;Jiao, John;Levchenko, Andre;Inoue, Takafumi;Rohatgi, Rajat;Inoue, Takanari
• 通讯作者: Inoue, Takanari

Lateral diffusion of inositol 1,4,5-trisphosphate receptor type 1 in Purkinje cells is regulated by calcium and actin filaments

• DOI: 10.1111/j.1471-4159.2010.06885.x
• 发表时间: 2010-09-01
• 期刊: JOURNAL OF NEUROCHEMISTRY
• 影响因子: 4.7
• 作者: Fukatsu, Kazumi;Bannai, Hiroko;Mikoshiba, Katsuhiko
• 通讯作者: Mikoshiba, Katsuhiko