Analysis of molecular dynamics in neuronal dendrites by simultaneous multi-point fluorescence correlation spectroscopy

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This research project aimed to measure dynamics of protein molecules rapidly moving in cytosole of neuronal dendrites at multiple points simultaneously by means of a newly developed two-photon microscope. I succeeded in recording at multiple points at more than 10 kHz time of resolution from cytosol of primary cultured neurons derived from mouse, in which GFP or GFP-fused CamKII protein was expressed, and in analyzing by FCS.