Establishment of evolutional microscopy on neurons with ultra-fast two-photon microscope

本研究项目旨在通过一种新开发的双光子显微镜同时测量在神经元树突胞质溶胶中快速移动的蛋白质分子的动力学。我成功地从表达GFP或GFP融合的CamKII蛋白的小鼠原代培养神经元的胞质溶胶中以超过10 kHz的时间分辨率在多个点进行记录，并通过FCS进行分析。我还成功地记录了来自多个培养神经元的动作电位，这使得能够分析神经元对之间的突触连接以及突触连接的方向。

This research project aimed to measure dynamics of protein molecules rapidly moving in the cytosol of neuronal dendrites at multiple points simultaneously by means of a newly developed two-photon microscope. I succeeded in recording at multiple points at more than 10 kHz of time resolution from cytosol of primary cultured neurons derived from mouse, in which GFP or GFP-fused CamKII proteins were expressed, and in analyzing by FCS. I was also successful in recording action potentials from multiple cultured neurons, which enabled to analyze synaptic connections between neuron pairs and also direction of synaptic connections.