Analysis of a gene function using ZFN and recombineering

使用 ZFN 和重组工程分析基因功能

• 批准号: 22650079
• 负责人: OHSAKO Shunji
• 金额: $ 2.13万
• 依托单位: Kyorin University (2011)Tokyo Metropolitan Organization for Medical Research (2010)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22650079/
• 关键词: ZFN; recombineering; CaMKII; dNZF; 遺伝子機能法; コンディショナル・ノックアウト; C2HC型Znフィンガー; ショウジョウバエ

A method for studying a gene function with complete control has been tried to develop by combinatorial use of a'zinc finger nuclease'(ZFN), which is a designed, sequence-specific endonuclease that can be customized to cleave a user-chosen DNA target, and recombineering, which is able to engineer large segments of genomic DNA of P[acman] for transgenesis in Drosophila melanogaster.

已经尝试通过使用a'Zinc指核酸酶（ZFN）结合使用A'Zinc Finger核酸酶来研究基因功能的一种方法，该方法是一种设计的，可以定制的，可以自定义的，可以自定义以裂解用户选择的DNA靶标，并重新组合型基因组MELAIN DNA [ACMAN DRAN] ACMANFEN [ACMAN] DRAN [ACMANS] dRAN [acMAN] dRAN [acMAN] dRAN [acMAN] dRAN [acMAN] acman drean dran Inshast [acman] acman indemand and insrast。

项目成果

Construction of CaMKII-interacting protein network obtained from affinity purified proteins in silico and its experimental verification.

从亲和纯化蛋白中获得的 CaMKII 相互作用蛋白网络的构建及其实验验证。

• 发表时间: 2010
• 作者: Yamada;T.;Iwamatsu;A.;Obayashi;T.;Kinoshita;K.;Ohsako;S.
• 通讯作者: S.

Drosophila C2HC-type Zn finger gene dnzf is required for differentiation of subsets of neurons.

果蝇 C2HC 型锌指基因 dnzf 是神经元亚群分化所必需的。

• 发表时间: 2010
• 作者: Ohsako;S.;Yamada;T.
• 通讯作者: T.