Establish a novel assay system to demonstrate the bioavailability of cancer preventive isothiocyanates in cruciferous vegetables by measuring its urinary metabolite

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Our goal is to establish a novel assay system to demonstrate the bioavailability of isothiocyanate by measuring its urinary metabolite, dithiocarbamate. However conventional methods cannot discriminate between dithiocarbamates derived from other cruciferous vegetables vs. test vegetable, thus requiring subjects to fast from cruciferous vegetables during the test. To avoid the inconvenience of fasting from cruciferous vegetables, we developed a novel HPLC method that can fractionate dithiocarbamates in test vegetable from other dithiocarbamates. To validate the assay, we determined levels of urinary dithiocarbamate from human subjects fed with test vegetable-derived isothiocyanate. The time needed for assessment is several days, and does not require the inconvenience of the fasting.