Development of the new method to control the molecular motor enzyme
Development of the new method to control the molecular motor enzyme
批准号:
22651048
负责人:
HISABORI Toru
金额:
$2.27万
依托单位国家:
日本
项目类别:
Grant-in-Aid for Challenging Exploratory Research
财政年份:
2010
资助国家:
日本
项目状态:
已结题
起止时间:
2010 至 2011
中文摘要
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英文摘要
Based on our hypothesis that the activity of ATP synthase can be regulated by the relative slippage of two alpha-helices of the gamma subunit, we intended to restrict the movement of two alpha-helices and studied the change in the enzyme activity. Then we tried to introduce a novel artificial switch into the gamma subunit to control the enzyme activity using a certain domain of light-responsible protein. In addition, the highly sensitive monitoring system to measure the enzyme activity at single molecule level at the aqueous phase was developed.
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会议论文
葉緑体ATP合成酵素の活性制御には、γサブユニットのα-ヘリックスのズレが関与する
γ 亚基的 α 螺旋错位参与调节叶绿体 ATP 合酶的活性
DOI:
--
发表时间:
2012
期刊:
影响因子:
--
作者:
[砂村栄一郎, 紺野宏記, 久堀徹]
通讯作者:
久堀徹
Target molecule recognition and redox regulation by the chloroplast thioredoxin
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批准号:17370015
-
项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$9.88万
-
财政年份:2005
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负责人:HISABORI Toru
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依托单位:
MOLECULAR MECHANISM OF REDOX REGULATION OF CHLOROPLAST ATP SYNTHSAE
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批准号:13440238
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$8.26万
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财政年份:2001
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负责人:HISABORI Toru
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依托单位:
Study on the regulatory region of the chloroplast ATP synthase by using peptide antibody
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批准号:11640643
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$2.3万
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财政年份:1999
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负责人:HISABORI Toru
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依托单位:
Assembly of Photosynthetic Apparatus on Cyanobacteria
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批准号:09044209
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项目类别:Grant-in-Aid for Scientific Research (B).
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资助金额:$3.84万
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财政年份:1997
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负责人:HISABORI Toru
-
依托单位:
Molecular Approach to the Regulatory Mechanism of Chloroplast ATP Synthase
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批准号:08640822
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$1.41万
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财政年份:1996
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负责人:HISABORI Toru
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依托单位:
海外基金