Development of NMR application to Geo-metabolomics

作为核磁共振在地球化学中的应用，我试图开发一种快速而简单的初步筛选方法来鉴定和定量地质样品(例如土壤和沉积物)提取物中的有机化合物。结果表明，1 mm探头的1H-MNR对于土壤和湖泊沉积物提取物中的叶绿素和氨基酸等目标有机化合物的快速、简便的初步筛选(包括鉴定和定量)是非常有用的。然而，目前，它对海洋沉积物中的萃取物还不够有效，因为这些样品中高浓度的有机酸和盐总是阻碍高分辨率的光谱扫描。此外，其他核磁共振如~lt；13&gt；^C和~lt；15&gt；N核磁共振，以及二维核磁共振，如1^H^&lt；-13&gt；C和1^H^&lt；-15&gt；N，由于光谱扫描的灵敏度较低，也不能作为地质样品的快速和简单的初步筛选方法。

As a NMR (Nuclear Magnetic Resonance) application to geochemistry, I tried to develop a rapid and simple primary screening method to identify and quantify organic compounds in extracts from geological samples (e.g., soils and sediments). The results show that 1H-MNR with 1 mm probe can be very much useful for a rapid and simple primary screening (both identification and quantification) of several targeted organic compounds such as chlorophylls and amino acids in the extracts from soils and lacustrine sediments. However, at this moment, it did not work sufficiently for extracts from marine sediments, because high concentration of organic acids and salts in these samples always obstruct spectrum-scan with high resolution. Moreover, other nuclear NMR such as <13>^C NMR and <15>^N NMR and two-dimensional NMR such as 1^H^<-13>C and 1^H^<-15>N were also not useful as a rapid and simple primary screening method for geological samples due to low sensitivity of the spectrum-scan.