Development of multi-stable isotope analytical method of amino acids

我开发了一种化合物特定的同位素方法来测量氨基酸的稳定氮、碳和氢同位素组成。结果表明：(1)甲氧基异丙酯衍生化可测定氮同位素组成，优于±0.5‰，最小进样量为0.5nmolN；(2)碳同位素组成可用乙酯衍生化测定，优于±0.5‰，最小进样量为0.1nmolN；氢同位素组成可用三乙酰/异丙酯衍生化测定，优于±5 0~10 0‰，最小进样量为0.5nmolN，但目前碳同位素分析仅适用于氨基酸标准溶液，不适用于天然样品。

I developed a compound-specific isotope method for measuring the stable nitrogen, carbon, and hydrogen isotopic compositions of amino acids. The results show(1) nitrogen isotopic composition can be determined by pivaloyl/isopropyl ester derivatization, with better than±0.5‰and the minimum sample amount of 0.5 nmol N,(2) carbon isotopic composition can be determined by ethyl ester derivatization, with better than±0.5‰and the minimum sample amount of 0.1 nmol N, and hydrogen isotopic composition can be determined by pivaloyl/isopropyl ester derivatization, with better than±50-100‰and the minimum sample amount of 0.5 nmol N. However, the carbon isotope analysis is only available for standard solution of amino acids but not for natural samples, at this moment.