Development of Artificial Plant Leafs using Polymeric Nanofibers and Formulation of Sugar-Production System

我们开发了一种利用LED照射大量培养植物细胞的新方法。将分离的植物细胞分散在乙烯乙酸乙烯酯共聚物（EVOH）溶液中。将该溶液用作静电纺丝的纺丝液，我们获得了固定在EVOH纤维毡上的植物细胞。我们证实了细胞在电纺后具有生物活性。将细胞分散在无糖培养基中，并在LED照射下培养一周。通过液相色谱质谱法（LC-MS）测试培养物，从而检测葡萄糖和蔗糖。这表明植物细胞中光合作用产生的糖被分泌到细胞壁外。另一方面，我们不能从含有固定在EVOH包埋垫上的细胞的培养物中检测到糖。这可能是由于（1）固定化细胞数量少;（2）必须用光合性能较高的细胞代替葛根细胞。

We developed a new method of mass cultivation for plant(Pueraria lobata) cell using LED irradiation. The isolated plant cells were dispersed in ethylene vinyl acetate copolymer(EVOH) solution. The solution was used as a spinning solution for electrospinning, and we obtained the plant cells immobilized on EVOH nanofiber mat. We confirmed that the cell had a bioactivity after the electrospinning.The cells were dispersed in a culture without sugar and it was cultured for a week under LED irradiation. The culture was tested by liquid chromatography mass spectrometry(LC-MS) and thus glucose and sucrose were detected. This indicates that sugar produced by photosynthesis in the plant cells was secreted to outside the cell walls. On the other hand, we could not detect sugar from the culture containing the cells immobilized on EVOH nanofiber mat. It is likely that(1) the cells immobilized were small in number ;(2) the cells with higher photosynthetic performance must be used instead of Pueraria lobata cells.