Development of a sensor for detection of pathogenic virus in water based on fluorescent molecule and virus binding protein

本研究的最终目标是开发一种用于检测水中致病性病毒的传感器。在本研究中，我们合成了病毒结合蛋白（VBP）和荧光团。VBP由转基因大肠杆菌产生。SDS-PAGE图谱显示，从大肠杆菌细胞中提取的可溶性蛋白为VBP，估计分子量为57 kDa。我们也可以在实验室合成荧光团（4,4 -二氟-4-硼-3a， 4-二氮-s-茚二烯）

The final goal of this study is to develop a sensor for detection of pathogenic virus in water. In this study, we synthesized virus-binding proteins(VBP) and fluorophore. VBP were produced by genetically-modified E. coli. The SDS-PAGE profile showed the extracted soluble proteins from E. coli cells were VBP of which the estimated molecular weight is 57 kDa. We could also synthesize fluorophore(4, 4-difluoro-4-bora-3a, 4a-diaza-s-indacene) in our laboratory