Development of a sensor for detection of pathogenic virus in water based on fluorescent molecule and virus binding protein

基于荧光分子和病毒结合蛋白的检测水中病原病毒的传感器的研制

• 批准号: 22656115
• 负责人: SATOH Hisashi
• 金额: $ 2.34万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22656115/
• 关键词: ウイルス; センサ; ウイルス吸着タンパク質; 蛍光分子; 光導波路; エバネッセント波; マイクロ流路; ウイルスセンサー; 環境分析; ナノ材料

The final goal of this study is to develop a sensor for detection of pathogenic virus in water. In this study, we synthesized virus-binding proteins(VBP) and fluorophore. VBP were produced by genetically-modified E. coli. The SDS-PAGE profile showed the extracted soluble proteins from E. coli cells were VBP of which the estimated molecular weight is 57 kDa. We could also synthesize fluorophore(4, 4-difluoro-4-bora-3a, 4a-diaza-s-indacene) in our laboratory

这项研究的最终目标是开发一种用于检测水中致病病毒的传感器。在这项研究中，我们合成了病毒结合蛋白（VBP）和荧光团。 VBP 由转基因大肠杆菌产生。 SDS-PAGE图谱显示从大肠杆菌细胞中提取的可溶性蛋白为VBP，其估计分子量为57 kDa。我们实验室还可以合成荧光团(4, 4-di氟-4-bora-3a, 4a-diaza-s-indacene)

项目成果

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