Development of a surface plasmon resonance sensorfor high throughput screening of O157

大肠杆菌可污染饮用水，对公共卫生安全构成重大威胁。为了防止这种严重的健康问题，鉴定和定量的细菌是非常重要的。目前，在过去的十年中，已经引入了各种有前途的方法来鉴定和量化水样中的病原体。然而，这些现有的技术很复杂，需要富集、荧光标记和较长的时间才能获得结果。因此，在现场环境中，甚至在家中，仍然需要快速和易于使用的水样病原体筛选工具。本研究的目的是建立一种快速检测液体样品中大肠杆菌O157:H7的SPR生物传感器。在20 min内检测出大肠杆菌O157:H7，传感器的检出限为10 ^ 8个功率电池/mL。甘氨酸-盐酸溶液再生传感器。

Escherichia coli (E. coli) can contaminate drinking water, posing a significant threat to public health safety. In order to prevent such serious health problems, identification and quantification of the bacteria is of great importance. At present, various promising approaches have been introduced over the past decade to identify and quantify pathogens in water samples. However, these existing techniques are complex and require enrichment, fluorescent labelling and longer time to obtain results. Thus, rapid and easy-to-use screening tools of pathogens in water samples are still needed in field settings or even at home. The objective of this study is to develop a SPR biosensor for rapid detection of E. coli O157:H7 in liquid samples. We could determined E. coli O157:H7 within 20 min. the detection limit of the sensor was 10 to the 8th power cells/mL. Glycine-HCl solution regenerated the sensor.