Histochemical electron microscopy to observe hydrogen peroxide distribution in the cells of crop plants

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Histochemical methods were developed to observe distribution of hydrogen peroxide in the cells of crop plants. In the CeCl3 method, leaf segments were incubated in 5 mM CeCl3 at pH 7. 2 for 1 h, fixed, dehydrated and embedded for electron microscopy. In the DAB method, leaf segments were fixed in Karnovski's fixative for 2 h and incubated in 10 mM DAB at pH 8. 0 for 1 h. After incubation the segments were prepared for electron microscopy. Hydrogen peroxide was detected around the grana of chloroplasts in salinity-treated rice plants.