Histochemical electron microscopy to observe hydrogen peroxide distribution in the cells of crop plants

采用组织化学方法观察了过氧化氢在作物细胞中的分布。在CeCl3方法中，叶段在pH为7的5 mM CeCl3中孵育。2放置1h，固定，脱水，包埋，用于电镜观察。在DAB法中，将叶段固定在Karnovski固定液中2 h，在pH为8的10 mM DAB中孵育。0孵育1 h。孵育后制备片段用于电镜观察。在盐碱化处理的水稻叶绿体颗粒周围检测到过氧化氢。

Histochemical methods were developed to observe distribution of hydrogen peroxide in the cells of crop plants. In the CeCl3 method, leaf segments were incubated in 5 mM CeCl3 at pH 7. 2 for 1 h, fixed, dehydrated and embedded for electron microscopy. In the DAB method, leaf segments were fixed in Karnovski's fixative for 2 h and incubated in 10 mM DAB at pH 8. 0 for 1 h. After incubation the segments were prepared for electron microscopy. Hydrogen peroxide was detected around the grana of chloroplasts in salinity-treated rice plants.