Molecular genetic analysis of ascorbate transport in plants

本研究通过筛选拟南芥抗坏血酸转运蛋白突变体来研究植物抗坏血酸转运机制。两种可能性最终在大约。8，000个EMS处理的M2拟南芥vtc种子，基于通过非光化学淬灭（NPQ）测量和用银的组织化学染色的组合的高通量筛选。此外，基于拟南芥T-DNA插入突变体中抗坏血酸摄取活性的计算机预测和实际评估的数据，表明至少有两个基因有可能是目的基因。

Screening of novel Arabidopsis mutants was carried out to identify ascorbate transport mechanism in plants. Two possibilities have finally obtained among approx. 8, 000 EMS-treated M2 Arabidopsis vtc seeds based on a high-throughput screening by a combination of non-photochemical quenching(NPQ) measurement and histochemical staining with silver nitrate. In addition, based on data from in silico prediction and practical evaluation of ascorbate up-take activity in Arabidopsis T-DNA inserted mutants, it has indicated that at least two genes have a possibility to be the target gene.