Molecular genetic analysis of ascorbate transport in plants

筛选新的拟南芥突变体，以确定抗坏血酸在植物中的转运机制。大约有两种可能性。基于非光化学猝灭（NPQ）测量和硝酸银组织化学染色相结合的高通量筛选，8000个ems处理的M2拟南芥vtc种子。此外，基于对拟南芥T-DNA插入突变体抗坏血酸摄取活性的计算机预测和实际评估数据，表明至少有两个基因有可能成为靶基因。

Screening of novel Arabidopsis mutants was carried out to identify ascorbate transport mechanism in plants. Two possibilities have finally obtained among approx. 8, 000 EMS-treated M2 Arabidopsis vtc seeds based on a high-throughput screening by a combination of non-photochemical quenching(NPQ) measurement and histochemical staining with silver nitrate. In addition, based on data from in silico prediction and practical evaluation of ascorbate up-take activity in Arabidopsis T-DNA inserted mutants, it has indicated that at least two genes have a possibility to be the target gene.