Molecular genetic analysis of ascorbate transport in plants

植物抗坏血酸转运的分子遗传学分析

• 批准号: 22658110
• 负责人: ISHIKAWA Takahiro
• 金额: $ 2.1万
• 依托单位: Shimane University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22658110/
• 关键词: 植物; アスコルビン酸輸送; バイオテクノロジー; 遺伝子; アスコルビン酸; 輸送調節機構; シロイヌナズナ; 輸送体; 代謝調節機構; 光合成生物

Screening of novel Arabidopsis mutants was carried out to identify ascorbate transport mechanism in plants. Two possibilities have finally obtained among approx. 8, 000 EMS-treated M2 Arabidopsis vtc seeds based on a high-throughput screening by a combination of non-photochemical quenching(NPQ) measurement and histochemical staining with silver nitrate. In addition, based on data from in silico prediction and practical evaluation of ascorbate up-take activity in Arabidopsis T-DNA inserted mutants, it has indicated that at least two genes have a possibility to be the target gene.

筛选新的拟南芥突变体，以确定抗坏血酸在植物中的转运机制。大约有两种可能性。基于非光化学猝灭（NPQ）测量和硝酸银组织化学染色相结合的高通量筛选，8000个ems处理的M2拟南芥vtc种子。此外，基于对拟南芥T-DNA插入突变体抗坏血酸摄取活性的计算机预测和实际评估数据，表明至少有两个基因有可能成为靶基因。

项目成果

シロイヌナズナvtc2変異体におけるアスコルビン酸取込み能の評価

拟南芥vtc2突变体抗坏血酸吸收能力的评价

• 发表时间: 2012
• 作者: 山本遥;孝田翔;丸田隆典;澤嘉弘;石川孝博
• 通讯作者: 石川孝博