Molecular genetic analysis of ascorbate transport in plants

为了确定植物体内抗坏血酸的运输机制，对拟南芥新突变体进行了筛选。大约有两种可能性终于实现了。通过非光化学猝灭(NPQ)测量和硝酸银组织化学染色相结合的高通量筛选，对8,000个经EMS处理的拟南芥M2种子进行了高通量筛选。此外，根据对拟南芥T-DNA插入突变体中抗坏血酸摄取活性的电子预测和实际评估的数据，表明至少有两个基因有可能成为靶基因。

Screening of novel Arabidopsis mutants was carried out to identify ascorbate transport mechanism in plants. Two possibilities have finally obtained among approx. 8, 000 EMS-treated M2 Arabidopsis vtc seeds based on a high-throughput screening by a combination of non-photochemical quenching(NPQ) measurement and histochemical staining with silver nitrate. In addition, based on data from in silico prediction and practical evaluation of ascorbate up-take activity in Arabidopsis T-DNA inserted mutants, it has indicated that at least two genes have a possibility to be the target gene.