Development of a novel gene expression system with antibody transcription factors

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A gene expression system has been used in the field of molecular biology because it enables genes of interest to express at desired period. However, only ligands for receptors derived from organisms are utilized, and compounds not to bind receptors are not suitable as a ligand. In this study, variable regions of the antibody toward polychlorinated biphenyls, DNA-binding domain, and transactivation domain were fused to construct an artificial transcription factor, we call antibody transcription factor. These fused proteins were produced in the recombinant yeast cells to create a novel gene expression system, which is applied for all compounds.