Development of Ornamental Plants for High Sensitive Monitoring of Persistent Organic Pollutants

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1. Development of the Receptor-mediated Supersensitive Monitoring System of POPsA. Supersensitive ReceptorsThe recombinant transcriptional factors including mouse (m), guinea pig (g), or human (h) aryl hydrocarbon receptors (XmDV, XgDV, XhDV) were constructed and introduced into tobacco and Arabidopsis plants together with the β-glucuronidase (GUS) reporter gene. When the transgenic plants were cultured with polychlorinated biphenyls (PCBs), induction of the GUS activity was dependent on PCB toxicity. The XmDV transgenic tobacco plants successfully detected dioxin contamination in soil.Transgenic Arabidopsis plants harboring the recombinant medaka estrogen receptor (ER) were obtained.B. POPs. Absorption, Metabolism, and Accumulation in PlantsThe zucchini cultivars that absorbed and accumulated 180 times more dioxins than tobacco plants were identified. We demonstrated that desorption of dioxins from soil matrices, absorption from surface of roots into cells, and translocation to aerial … More parts were important for dioxin absorption in the zucchini, and that citric acid was responsible for high accumulation of dioxins.C. Signal Transduction System for POPs ContaminationNovel recombinant transcriptional factors including movement protein (MP) that was involved in intercellular transporting of viruses in plant cells were introduced into the XgDV transgenic Arabidopsis plants. The GUS activity in the flower stalks of the MP/XgDV transgenic plants treated with PCB126 significantly increased, therefore the dioxin contamination signal was successfully transmitted to the aerial parts of the plants.2. Development of Transgenic Ornamental Plants for Monitoring of POPsA. Transformation of Petunia, Torenia, and VerbenaTransgenic ornamental plants harboring the XmDV, XgDV, or ER, and RNAi of flower color synthesis genes were obtained.B. Monitoring of Dioxin Contaminated Soil Using the Transgenic Ornamental PlantsA part of the flower color changed in the transgenic ornamental plants that were cultivated in the dioxin contaminated soil, but more research needs to be done for stable monitoring. Less