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Development of Ornamental Plants for High Sensitive Monitoring of Persistent Organic Pollutants

开发用于高灵敏度监测持久性有机污染物的观赏植物

基本信息

批准号：
17208029
负责人：
INUI Hideyuki
金额：
$ 31.28万
依托单位：
Kobe University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2007
项目状态：
已结题

项目摘要

1. Development of the Receptor-mediated Supersensitive Monitoring System of POPsA. Supersensitive ReceptorsThe recombinant transcriptional factors including mouse (m), guinea pig (g), or human (h) aryl hydrocarbon receptors (XmDV, XgDV, XhDV) were constructed and introduced into tobacco and Arabidopsis plants together with the β-glucuronidase (GUS) reporter gene. When the transgenic plants were cultured with polychlorinated biphenyls (PCBs), induction of the GUS activity was dependent on PCB toxicity. The XmDV transgenic tobacco plants successfully detected dioxin contamination in soil.Transgenic Arabidopsis plants harboring the recombinant medaka estrogen receptor (ER) were obtained.B. POPs. Absorption, Metabolism, and Accumulation in PlantsThe zucchini cultivars that absorbed and accumulated 180 times more dioxins than tobacco plants were identified. We demonstrated that desorption of dioxins from soil matrices, absorption from surface of roots into cells, and translocation to aerial … More parts were important for dioxin absorption in the zucchini, and that citric acid was responsible for high accumulation of dioxins.C. Signal Transduction System for POPs ContaminationNovel recombinant transcriptional factors including movement protein (MP) that was involved in intercellular transporting of viruses in plant cells were introduced into the XgDV transgenic Arabidopsis plants. The GUS activity in the flower stalks of the MP/XgDV transgenic plants treated with PCB126 significantly increased, therefore the dioxin contamination signal was successfully transmitted to the aerial parts of the plants.2. Development of Transgenic Ornamental Plants for Monitoring of POPsA. Transformation of Petunia, Torenia, and VerbenaTransgenic ornamental plants harboring the XmDV, XgDV, or ER, and RNAi of flower color synthesis genes were obtained.B. Monitoring of Dioxin Contaminated Soil Using the Transgenic Ornamental PlantsA part of the flower color changed in the transgenic ornamental plants that were cultivated in the dioxin contaminated soil, but more research needs to be done for stable monitoring. Less

1. POPsA受体介导的超灵敏监测系统的建立超敏感受体构建了含有小鼠（m）、豚鼠（g）和人（h）芳烃受体的重组转录因子（XmDV、XgDV、XhDV），并将其与β-葡萄糖醛酸酶（GUS）报告基因一起导入烟草和拟南芥植株中。当转基因植物与多氯联苯（PCB）一起培养时，GUS活性的诱导取决于PCB毒性。利用XmDV转基因烟草成功检测了土壤中二恶英污染，获得了转基因拟南芥，并获得了重组青鳉雌激素受体（ER）基因。pops.植物的吸收、代谢和积累西葫芦品种吸收和积累的二恶英比烟草植物多180倍。研究表明，二恶英从土壤基质中解吸，从根表面吸收到细胞中，再转移到空气中， ...更多信息部分是西葫芦吸收二恶英的重要因素，柠檬酸是西葫芦积累二恶英的主要原因。持久性有机污染物污染的信号转导系统将参与病毒在植物细胞中胞间转运的新型重组转录因子运动蛋白（Movement protein，MP）导入XgDV转基因拟南芥植株中。PCB126处理的MP/XgDV转基因植株花柄中GUS活性显著提高，二恶英污染信号成功传递到植株地上部分.利用转基因观赏植物监测持久性有机污染物。矮牵牛、蓝猪耳和马鞭草的转化获得了携带XmDV、XgDV或ER的转基因观赏植物，以及花色合成基因的RNAi。利用转基因观赏植物监测二恶英污染土壤转基因观赏植物在二恶英污染土壤中栽培后，部分花色发生了变化，但稳定监测还需进一步研究。少