Development of Ornamental Plants for High Sensitive Monitoring of Persistent Organic Pollutants

1. 受体介导的POPsA超灵敏监测系统的研制。构建小鼠(m)、豚鼠(g)和人(h)芳烃受体（XmDV、XgDV、XhDV）重组转录因子，并将其与β-葡萄糖醛酸酶（GUS）报告基因一起导入烟草和拟南芥植株。当转基因植株与多氯联苯（PCB）一起培养时，GUS活性的诱导取决于多氯联苯的毒性。转基因烟草成功检测出土壤中的二恶英污染。获得了含有重组medaka雌激素受体（ER）的转基因拟南芥植株。持久性有机污染物。植物对二恶英的吸收、代谢和积累经鉴定，西葫芦品种对二恶英的吸收和积累比烟草多180倍。研究结果表明，西葫芦对二恶英的吸收主要通过土壤基质的解吸、根表面对细胞的吸收以及向空气中的转运等途径进行，其中柠檬酸对二恶英的积累起着重要作用。POPs污染的信号转导系统将包括运动蛋白（MP）在内的新型重组转录因子引入到XgDV转基因拟南芥植株中。PCB126处理后，MP/XgDV转基因植株花茎中GUS活性显著升高，表明二恶英污染信号成功传递到植株的空中部位。用于监测POPsA的转基因观赏植物的开发。获得了含有XmDV、XgDV或ER的牵牛花、托伦尼亚和马尾草转基因观赏植物的转化和花色合成基因的RNAi。利用转基因观赏植物对二恶英污染土壤的监测转基因观赏植物在二恶英污染土壤中栽培后，其花的颜色发生了部分变化，但为了实现稳定的监测，还需要做更多的研究。少

1. Development of the Receptor-mediated Supersensitive Monitoring System of POPsA. Supersensitive ReceptorsThe recombinant transcriptional factors including mouse (m), guinea pig (g), or human (h) aryl hydrocarbon receptors (XmDV, XgDV, XhDV) were constructed and introduced into tobacco and Arabidopsis plants together with the β-glucuronidase (GUS) reporter gene. When the transgenic plants were cultured with polychlorinated biphenyls (PCBs), induction of the GUS activity was dependent on PCB toxicity. The XmDV transgenic tobacco plants successfully detected dioxin contamination in soil.Transgenic Arabidopsis plants harboring the recombinant medaka estrogen receptor (ER) were obtained.B. POPs. Absorption, Metabolism, and Accumulation in PlantsThe zucchini cultivars that absorbed and accumulated 180 times more dioxins than tobacco plants were identified. We demonstrated that desorption of dioxins from soil matrices, absorption from surface of roots into cells, and translocation to aerial … More parts were important for dioxin absorption in the zucchini, and that citric acid was responsible for high accumulation of dioxins.C. Signal Transduction System for POPs ContaminationNovel recombinant transcriptional factors including movement protein (MP) that was involved in intercellular transporting of viruses in plant cells were introduced into the XgDV transgenic Arabidopsis plants. The GUS activity in the flower stalks of the MP/XgDV transgenic plants treated with PCB126 significantly increased, therefore the dioxin contamination signal was successfully transmitted to the aerial parts of the plants.2. Development of Transgenic Ornamental Plants for Monitoring of POPsA. Transformation of Petunia, Torenia, and VerbenaTransgenic ornamental plants harboring the XmDV, XgDV, or ER, and RNAi of flower color synthesis genes were obtained.B. Monitoring of Dioxin Contaminated Soil Using the Transgenic Ornamental PlantsA part of the flower color changed in the transgenic ornamental plants that were cultivated in the dioxin contaminated soil, but more research needs to be done for stable monitoring. Less