Bioinformation Produced by Molecular Interactions in The Membrane Microdomains

为了改进最近建立的分析质膜中分子相互作用的方法EMARS，我们开发了第二代标记试剂，并利用基于质谱学的蛋白质组学技术来鉴定EMARS方法标记的蛋白质。此外，我们还发现在细胞外基质蛋白或治疗性抗体的刺激下，质膜中的分子相互作用发生了变化，并阐明了新的分子参与了导致生物效应的信号通路。

In order to improve the recently established method to analyze molecular interactions in the plasma membranes, called EMARS, we developed the second generation labeling reagent and enabled to identify proteins labeled by the EMARS method by using mass spectrometry-based proteomics technology. Furthermore, we found changes in molecular interactions in the plasma membranes elicited by stimulations with extracellular matrix proteins or therapeutic antibodies and elucidated the involvement of novel molecules in the signal pathway leading to the biological effects.