Towards development of a novel low-temperature plasma sterilizer : effects of nitrogen gas plasma on bacterial macromolecules and toxins.

开发新型低温等离子体灭菌器：氮气等离子体对细菌大分子和毒素的影响。

• 批准号: 22659085
• 负责人: KIDO Nobuo
• 金额: $ 1.84万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22659085/
• 关键词: 低温滅菌; プラズマ; 大腸菌; ブドウ球菌; エンドトキシン; 滅菌; 低温ガスプラズマ; リポ多糖体; 窒素ラジカル; 高電圧パルス; 紫外線; Geobacillus stearothermophilus; Staphylococcus aureus; Escherichia coli; D値; 滅菌保証時間

To develop a novel low-temperature plasma sterilizer using purenitrogen(N_2) gas as a plasma source, we evaluated bactericidal ability of a prototype apparatus. The decimal reduction value of the biological indicator of Geobaclllus stearothermophilus endospores was determined as 1. 9 min. Staphylococcus aureus and Escherichia coil were also exposed to the N2 gas plasma and confirmed to be inactivated within 30 min. Through the evaluation of bactericidal efficiency in a sterilization bag, we concluded that the UV photons in the plasma and the high voltage pulse to generate the gas plasma were not concerned with the bactericidal effect of the N2 gas plasma. Bactericidal effect might be exhibited by activated nitrogen atoms or molecular radicals. Endotoxins from Gram-negative bacteria were inactivated more efficiently than the autoclave treatment. Moreover, the biological activities of endotoxins also decreased significantly. The Ames test indicated that mutations induced by the plasma irradiation were negligible.

为了研制一种新型的纯氮低温等离子体灭菌器，对一台样机的杀菌能力进行了评价。生物指示剂嗜热脂肪土芽孢杆菌内生孢子的十进制减少值为1。金黄色葡萄球菌和大肠杆菌也暴露于N2气体等离子体中，并证实在30 min内被灭活。通过对灭菌袋中杀菌效率的评价，我们得出结论，等离子体中的紫外光子和产生气体等离子体的高压脉冲与N2气体等离子体的杀菌效果无关。杀菌作用可能通过活化的氮原子或分子自由基来表现。来自革兰氏阴性菌的内毒素比高压灭菌处理更有效地灭活。此外，内毒素的生物活性也显著降低。艾姆斯试验表明，等离子体辐照诱发的突变可忽略不计。

项目成果

Evaluation of bactericidal effects of low‐temperature nitrogen gas plasma towards application to short‐time sterilization

• DOI: 10.1111/j.1348-0421.2012.00457.x
• 发表时间: 2012-07
• 期刊: Microbiology and Immunology
• 影响因子: 2.6
• 作者: K. Kawamura;Ayaka Sakuma;Yuka Nakamura;Tomoko Oguri;Natsumi Sato;N. Kido

新規滅菌装置の開発:低温窒素ガスプラズマを用いた病原細菌の滅菌

新型灭菌装置的开发：利用低温氮气等离子体对病原菌进行灭菌

• 发表时间: 2011
• 作者: 川村久美子;小栗智子;佐藤夏巳;新谷英晴;今西雄一郎;清水尚博;木藤伸夫
• 通讯作者: 木藤伸夫