Non-invasive assessment for therapeutic response to gene therapy using redox imaging and MALDI-TOF mass-spectrometry

点击翻译按钮获取中文摘要

The purpose of this study is to develop the non-invasive assays to evaluate therapeuticresponse to gene therapy, as the indexes of redox state assessed by Overhauser MRI(OMRI) and metabolic profiling analyzed by MALDI-TOF mass-spectrometry. The ratio of radical disappearance curve analyzed by OMRI was increased for the TNF-αgene therapy compared with the control in subcutaneous tumor model. However, there was no significant difference between two groups in peritoneal dissemination, wherethe heterogeneity of tumor and normal tissues was present, suggesting the gating technology of redox imaging restricting tumor area may be necessary to improve the assay. In a separate experiment, the microanalysis of metabolites in endothelial cells post-transfection of redox-related transcription factor: YB-1-targeting siRNA using MALDI-TOF mass-spectrometry revealed the reduction of NAD and the increase of Histidine and Aspartate in cells with YB-1-siRNA. These results suggest that the improvement of visualizing (gating) ability of OMRI for tumor area could potentiate the redox imaging-based non-invasive assay to estimate the response to gene therapy.