A new method for stress assessment using stress-specific alternative splice variants

点击翻译按钮获取中文摘要

Alternative splicing events in healthy medical students challenging an examination for promotion were analyzed using exon array and real-time RT-PCR. We identified the stress-initiated skipping of exon 63 of SMG-1 that encodes a phosphatidylinositol 3-kinase-related protein kinase crucial for activations of p53-dependent pathways(Neurosci Lett 2010). Using National Medical License Examination as a chronic psychological stress model, we also examined alternative splicing of the glucocorticoid receptor(GR) gene in medical students, and found that the persistant stressful situations specifically decreased a non-functional isoform(GR-beta) in medical students(Stress 2011).