A study on cell-fate switching between megakaryocytes and adipocytes in bone marrow cells

骨髓细胞巨核细胞与脂肪细胞命运转换的研究

• 批准号: 22659183
• 负责人: IKEDA Yasuo
• 金额: $ 2.11万
• 依托单位: Waseda University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22659183/
• 关键词: 血栓止血学; 再生医療; 血小板; 巨核球; 骨髄脂肪変性; 造血幹細胞; 間葉系幹細胞; 脂肪前駆細胞; 分化誘導; 細胞運命制御; 細胞分化運命制御

We carried out screening of megakaryocyte-inducing factors based on our previous findings ; megakaryocytes and platelets were differentiated from preadipocyte cell line 3T3-L1, but not its parent cell line 3T3 fibroblasts. Gene expression analysis of candidate transcription factors and gene transfection study showed that p45NF-E2, Maf G, and Maf K are determinant factors for megakaryocyte differentiation and platelet production. Megakaryocyte differentiation of p45NF-E2/Maf G/Maf K-expressing fibroblasts was inhibited by the co-expression of CEBP alpha, known to be a determinant factor for adipocytes. Thus, p45NF-E2/Maf G/Maf K and CEBPalpha are critical factors in cell-fate swiching between megakaryocytes and adipocytes.

我们根据之前的研究结果进行了巨核细胞诱导因子的筛选；巨核细胞和血小板可从前脂肪细胞系3T3- l1分化，但不能从其亲本细胞系3T3成纤维细胞分化。候选转录因子的基因表达分析和基因转染研究表明，p45NF-E2、Maf G和Maf K是巨核细胞分化和血小板产生的决定因子。表达p45NF-E2/Maf G/Maf k的成纤维细胞的巨核细胞分化被CEBP α的共同表达所抑制，CEBP α是脂肪细胞的决定因素。因此，p45NF-E2/Maf G/Maf K和CEBPalpha是巨核细胞和脂肪细胞之间细胞命运转换的关键因素。

项目成果

Generation of Megakaryocytes from Mouse Subcutaneous Adipose Tissues in vitro.

体外从小鼠皮下脂肪组织中产生巨核细胞。

• 发表时间: 2010
• 作者: Akira Sonoda;Yumiko Matsubara;Hidenori Suzuki;Yasuo Ikeda
• 通讯作者: Yasuo Ikeda

GSK-3beta negatively regulates megakaryocyte differentiation and platelet production from human bone marrow cells in vitro.

GSK-3beta 在体外负调节人骨髓细胞的巨核细胞分化和血小板生成。

• 发表时间: 2010
• 期刊: Platelets
• 影响因子: 3.3
• 作者: Mayumi Ono;Yumiko Matsubara;Yasuo Ikeda;Mitsuru Murata
• 通讯作者: Mitsuru Murata

Generation of Megakaryocytes from Mouse Subcutaneous Adipose Tissues in vitro

体外从小鼠皮下脂肪组织中产生巨核细胞

• 发表时间: 2010
• 作者: Sonoda A;Matsubara Y;Suzuki H;Ikeda Y;Matsuo K;Murata M
• 通讯作者: Murata M

GSK-3beta negatively regulates megakaryocyte differentiation and platelet production from human bone marrow cells in vitro

GSK-3beta 负调节人骨髓细胞体外巨核细胞分化和血小板生成

• 发表时间: 2011
• 期刊: Platelets
• 影响因子: 3.3
• 作者: Ono M;Matsubara Y;Shibano Y;Ikeda Y;Murata M
• 通讯作者: Murata M

Platelets and Megakaryocytes, Methods and Protocols

血小板和巨核细胞、方法和方案

• 发表时间: 2011
• 作者: Yuuiko Matsubara;Mitsuru Nurata;Yasuo Ikeda
• 通讯作者: Yasuo Ikeda