Comprehensive analysis for epigenetic modulation of the effects of anticancer agents on cancer stem cells

抗癌药物对癌症干细胞影响的表观遗传调控综合分析

• 批准号: 22659233
• 负责人: UTSUNOMIYA Tohru
• 金额: $ 2.07万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22659233/
• 关键词: エピジェネティック修飾; がん幹細胞; 幹細胞マーカー; ヒストン脱アセチル化; 抗癌剤抵抗性; 分化誘導; DNAマイクロアレイ解析; ネットワーク解析

We investigated the significance of epigenetic modulation of the effects of anticancer agents on cancer stem cells. We found that combination therapy of anticancer agents and histone deacetylase inhibitor(HDACi) markedly reduced cancer cell viability compared to that of each single agent alone. We confirmed that HDACi induced cell differentiation of cancer cell lines in terms of stemness genes and cancer stem cell markers. Finally, we identified the candidate genes, which might be responsible to the synergistic effects of HDACi, using a DNA microarray analysis and a gene network analysis.

我们研究了抗癌药物对癌症干细胞作用的表观遗传调控的意义。我们发现抗癌药物和组蛋白去乙酰化酶抑制剂（HDACi）联合治疗比单独使用单个药物显著降低癌细胞活力。我们从干细胞基因和肿瘤干细胞标记物方面证实了HDACi诱导肿瘤细胞系的细胞分化。最后，我们利用DNA微阵列分析和基因网络分析确定了可能与HDACi协同作用有关的候选基因。

项目成果

Modified hanging method for liver resection and liver mobilization

改良悬挂法肝切除及肝脏动员

• DOI: 10.1007/s00534-011-0442-1
• 发表时间: 2011
• 期刊: J Hepatobiliary Pancreat Surg
• 作者: Utsunomiya T;et al.(2人中1番目)
• 通讯作者: et al.(2人中1番目)

Hypoxia Inducible Factor Expression in Intrahepatic Cholangiocarcinoma

• DOI: 10.5754/hge11156
• 发表时间: 2011-09-01
• 期刊: HEPATO-GASTROENTEROLOGY
• 作者: Morine, Yuji;Shimada, Mitsuo;Miyake, Hidenori
• 通讯作者: Miyake, Hidenori

Role of histone deacetylase expression in IHCC : A possible epigenetic regulation of cancer stem cell

组蛋白脱乙酰酶表达在 IHCC 中的作用：癌症干细胞的可能表观遗传调控

• 发表时间: 2011
• 作者: Utsunomiya T;et al.(10人中3番目)
• 通讯作者: et al.(10人中3番目)

Expression of thrombospondin-1 and Ski are prognostic factors in advanced gastric cancer

• DOI: 10.1007/s10147-010-0147-5
• 发表时间: 2011-04-01
• 期刊: INTERNATIONAL JOURNAL OF CLINICAL ONCOLOGY
• 影响因子: 3.3
• 作者: Nakao, Toshihiro;Kurita, Nobuhiro;Shimada, Mitsuo
• 通讯作者: Shimada, Mitsuo

Effect of histone deacetylase inhibitor in combination with 5-fluorouracil on pancreas cancer and cholangiocarcinoma cell line

组蛋白脱乙酰酶抑制剂联合5-氟尿嘧啶对胰腺癌和胆管癌细胞系的影响

• 发表时间: 2011
• 作者: Utsunomiya T;et al.(12人中3番目)
• 通讯作者: et al.(12人中3番目)