Generation of intestinal epithelial cell line from induced pluripotent stem cell and establishment of colorectal cancer stem cell model

诱导多能干细胞产生肠上皮细胞系及结直肠癌干细胞模型的建立

• 批准号: 22659242
• 负责人: HINOI Takao
• 金额: $ 2.07万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22659242/
• 关键词: 小腸大腸肛門外科学; iPS細胞; 腸上皮細胞; 大腸癌幹細胞

Induced pluripotent stem cell is the ideal stem cell and it is not necessary to use embryonic stem cell. In this study, we aimed to establish normal intestinal epithelium cell line by progenitor of endoderm induced from mouse iPS cells in the first step and differentiated by gene transfer of intestine-specific homeobox transcription factor CDX2. In the next step, we aim to generate iPS-induced colorectal cancer stem cell line with transformation using gene transfer of colorectal cancer-related gene. By analyzing the these cell lines, we aimed to identify novel target of the colorectal cancer-related genes with the microarray analysis.

诱导多能干细胞是理想的干细胞，不一定要使用胚胎干细胞。在本研究中，我们首先从小鼠iPS细胞诱导内胚层祖细胞，并通过肠道特异性同源盒转录因子CDX2的基因转移来诱导分化，以建立正常的肠上皮细胞系。下一步，我们的目标是利用结直肠癌相关基因的基因转移，建立iPS诱导的结直肠癌干细胞系。通过对这些细胞系的分析，我们旨在通过微阵列分析寻找结直肠癌相关基因的新靶点。

项目成果

Identification of patients likely to benefit from metastasectomy in stage IV colorectal cancer

• DOI: 10.1007/s00384-012-1454-2
• 发表时间: 2012-10-01
• 期刊: INTERNATIONAL JOURNAL OF COLORECTAL DISEASE
• 影响因子: 2.8
• 作者: Shimomura, Manabu;Okajima, Masazumi;Ohdan, Hideki
• 通讯作者: Ohdan, Hideki

External validation of two nomograms for predicting patient survival after hepatic resection for metastatic colorectal cancer

用于预测转移性结直肠癌肝切除术后患者生存率的两个列线图的外部验证

• 发表时间: 2011
• 期刊: World J Surg
• 影响因子: 2.6
• 作者: Takakura Y;Okajima M;Kanemitsu Y;Kuroda S;Egi H;Hinoi T;Tashiro H;Ohdan H
• 通讯作者: Ohdan H

Adequate lymph node examination is essential to ensure the prognostic value of the lymph node ratio in patients with stage III colorectal cancer

• DOI: 10.1007/s00595-010-4446-2
• 发表时间: 2011-10-01
• 期刊: SURGERY TODAY
• 影响因子: 2.5
• 作者: Shimomura, Manabu;Ikeda, Satoshi;Ohdan, Hideki
• 通讯作者: Ohdan, Hideki