Generation of intestinal epithelial cell line from induced pluripotent stem cell and establishment of colorectal cancer stem cell model

诱导性多能干细胞是理想的干细胞，不一定要使用胚胎干细胞。本研究的目的是通过小鼠iPS细胞初步诱导内胚层祖细胞，并通过肠特异性同源盒转录因子CDX 2的基因转移进行分化，建立正常肠上皮细胞系。下一步，我们的目标是利用基因转移技术建立诱导的大肠癌干细胞系。通过分析这些细胞系，我们的目的是确定新的目标大肠癌相关基因的芯片分析。

Induced pluripotent stem cell is the ideal stem cell and it is not necessary to use embryonic stem cell. In this study, we aimed to establish normal intestinal epithelium cell line by progenitor of endoderm induced from mouse iPS cells in the first step and differentiated by gene transfer of intestine-specific homeobox transcription factor CDX2. In the next step, we aim to generate iPS-induced colorectal cancer stem cell line with transformation using gene transfer of colorectal cancer-related gene. By analyzing the these cell lines, we aimed to identify novel target of the colorectal cancer-related genes with the microarray analysis.