Development of drug delivery system for central nervous system disease using with temperature control devise

点击翻译按钮获取中文摘要

Drug delivery system(DDS) through blood brain barrier(BBB) promises to enhance the effectiveness of medicine for nervous system diseases such as epilepsy and malignant brain tumors. In order to develop the DDS system, control of BBB permeability of molecules by alteration of temperature was analyzed. Tight junction of artificial BBB made from mouse endothelial cell was temporary opened at 15 degrees centigrade. And claudin5, one of the major structural proteins of the tight junction decreased the amount of expression at 15 degrees. Evans blue administered to a rat vein leaked to the brain surface when it was cooled. The increased permeability was reversible by the temperature control. Indocyanine green administered intravenously to an epilepsy patient appeared to the cooled area of brain surface at the surgery of temporal lobectomy. These results lead that the temperature control may modulate BBB permeability in a reversible fashion.