Regulatory mechanisms of osteoclast apoptosis by Bim and Puma

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The Bcl-2 family proteins are known as key regulators of apoptosis. In osteoclasts, Bcl-2 family proteins were reported to regulate not only cell viability but also bone resorbing activity. To determine the role of pro-apoptotic Bcl-2 family proteins Bim and Puma, we made knock-out mouse and evaluate bone morphometry, osteoclast viability and bone resorbing activity. Bim KO mice showed mild osteosclerosis due to impaired bone resorption, despite their osteoclast survival were extended. Puma KO mouse had no phenotype in bone morphometry. Puma KO osteoclast had extended viability but there were no significant difference in bone resorbing activity.Making Bim and Puma double knock-out mouse(Bim-/-Puma-/-) are required for further understanding of the co-regulation between Bim and Puma in osteoclast.