Functional relation of stress response in blood cells and brain after hypoxia

生物应激决定着从事故和手术等紧急情况下的恢复，并影响细胞内的信号转导。甘油二酯激酶(DGK)是肌醇磷脂代谢过程中代谢第二信使DG的一种酶。最近的研究表明，二酰甘油激酶(DGK)参与了多种疾病，如癌症、糖尿病、炎症和中风。在目前的研究中，我们在生物体水平上进行了一项实验，使用的是缺氧室应激(氧气浓度为5%)。在短暂的低氧应激3天后，我们发现DGKζ从胞核移位到胞浆。这些结果为DGKζ的核浆易位提供了证据，可能是细胞凋亡早期的一个指标。

Biological stress determines recovery from emergency conditions, such as accidents and surgery and affects intracellular signal transduction. Diacylglycerol kinase (DGK) is an enzyme that metabolizes the second messenger DG in phosphoinositide turnover. Recent studies have indicated an involvement of diacylglycerol kinase (DGK) in various diseases such as cancer, diabetes, inflammation and stroke. In the present study we performed an experiment at the organismal level using hypoxic chamber stress (oxygen concentration 5%). After 3 days of transient hypoxic stress, we found that DGKζ was translocated from the nucleus to the cytoplasm. These results provide an evidence that nucleo-cytoplasmic translocation of DGKζ may be an index of early phase of apoptosis.