Artificial gingival composed by regenerated epithelia and bone from stem cells

由干细胞再生上皮和骨组成的人工牙龈

• 批准号: 22659384
• 负责人: YAEGAKI Ken
• 金额: $ 2.14万
• 依托单位: The Nippon Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22659384/
• 关键词: 上皮; 幹細胞; 角化細胞; インテグリン; CD71; 歯肉; 基底膜; integrin; keratin19; p63; keratin 19; 細胞表面マーカー

Although oral keratinocyte stem cells play a key role in tissue homeostasis, wound healing, and neoplasia, they remain difficult to identify and characterize. The specific aim of the present study is to characterize an oral keratinocyte stem-cell population separated using a magnetic technique. Oral human keratinocytes obtained from keratinized oral mucosa were magnetically separated using a proliferation-related marker, CD71 and α6ss4 integrin. The expression of different stem cell markers: CD44H, Nestin,Nanog, Oct 3/4, CD117 was checked by immunofluorescence. The ability of α6ss4pos CD71neg fraction to form oral epithelial equivalents was also assayed. Three different oral keratinocyte subpopulations were obtained following magnetic separation: α6ss4pos CD71neg,α6ss4pos CD71pos and α6ss4neg. Our α6ss4pos CD71neg stem cell fraction was positive for Oct 3/4, CD44H and cytokeratin 19 while Nanog, Nestin and CD117 expression was absent. At the same time, the other two cell fractions α6ss4pos CD71pos and α6ss4neg were negative for all stem cell markers. Also, α6ss4pos CD71neg fraction was able to regenerate a well stratified and organized oral epithelial equivalent. The distribution of cytokeratin 19 and involucrin in the oral epithelial equivalent reflected the in vivo situation in oral gingival epithelium. It is also suggested that a magnetic system may be an important tool in acquiring oral keratinocyte stem cells for research.

虽然口腔角质形成细胞干细胞在组织稳态、伤口愈合和肿瘤形成中起关键作用，但它们仍然难以识别和表征。本研究的具体目的是表征口腔角质形成细胞干细胞群分离使用磁性技术。使用增殖相关标记物CD 71和α 6 β 4整联蛋白对从角化口腔粘膜中获得的口腔人角化细胞进行磁性分离。通过免疫荧光检测不同干细胞标志物：CD 44 H、Nestin、Nanog、Oct 3/4、CD 117的表达。还测定了α 6 β 4 pos CD 71阴性组分形成口腔上皮等同物的能力。经磁分离后获得3个不同的口腔角质形成细胞亚群：α 6 ss 4pos CD 71 neg、α 6 ss 4pos CD 71 pos和α 6 ss 4 neg。我们的α 6 ss 4 pos CD 71阴性干细胞部分对Oct 3/4、CD 44 H和细胞角蛋白19呈阳性，而Nanog、Nestin和CD 117表达缺失。同时，其他两种细胞组分α 6 ss 4 pos CD 71 pos和α 6 ss 4 neg对所有干细胞标志物均呈阴性。此外，α 6 ss 4pos CD 71阴性组分能够再生分层良好且组织化良好的口腔上皮等同物。细胞角蛋白19和外皮蛋白在牙龈上皮中的分布反映了牙龈上皮的生物学特性。也有人建议，磁系统可能是一个重要的工具，在获取口腔角质形成干细胞的研究。

项目成果

Oral keratinocyte stem cells and oral epithelial equivalents

口腔角质形成细胞干细胞和口腔上皮细胞等同物

• 发表时间: 2011
• 作者: 植田紘貴;八木孝和;菅真有;宮脇正一;Calenic B
• 通讯作者: Calenic B

Characterization of oral keratinocyte stem cells and prospects of its differentiation to oral epithelial equivalents.

• 发表时间: 2010
• 期刊: Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie
• 作者: B. Calenic;N. Ishkitiev;Ken Yaegaki;Toshio Imai;M. Costache;Mihaela Tovaru;Ș. Tovaru;I. Parlatescu

p53-Pathway activity and apoptosis in hydrogen sulfide-exposed stem cells separated from human gingival epithelium

• DOI: 10.1111/jre.12011
• 发表时间: 2013-06-01
• 期刊: JOURNAL OF PERIODONTAL RESEARCH
• 影响因子: 3.5
• 作者: Calenic, B.;Yaegaki, K.;Tanaka, T.
• 通讯作者: Tanaka, T.

Magnetic separation and characterization of keratinocyte stem cells from human gingiva

• DOI: 10.1111/j.1600-0765.2010.01284.x
• 发表时间: 2010-12-01
• 期刊: JOURNAL OF PERIODONTAL RESEARCH
• 影响因子: 3.5
• 作者: Calenic, B.;Ishkitiev, N.;Hirata, T.
• 通讯作者: Hirata, T.

Didilescu A Oral keratinocyte stem cells and oral epithelial equivalents, The 45th Meeting of Continental European Division of IADR, Budapest

Didilescu A 口腔角质形成细胞干细胞和口腔上皮等同物，IADR 欧洲大陆分部第 45 届会议，布达佩斯

• 发表时间: 2011
• 作者: Calenic B;Yaegaki K;Greabu M
• 通讯作者: Greabu M