Study for the Process of Developing General Conditions Because of Periodontitis: Effects of Volatile Sulfur Compounds on Stem Cells

牙周炎一般病症发生过程的研究：挥发性硫化合物对干细胞的影响

• 批准号: 20390538
• 负责人: YAEGAKI Ken
• 金额: $ 11.98万
• 依托单位: The Nippon Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20390538/
• 关键词: 歯周炎; 幹細胞; 肝細胞; 硫化水素; 口臭皮; 活性酸素; 肝臓様細胞; 再生医療; 口臭; アポトーシス; ROS; 胚細胞; 上皮細胞; 歯髄

Stem cells were isolated from human deciduous and wisdom teeth using CD117 antibody and magnetic separation. The cells were passaged using the magnetic separation using CD117 antibody at every 4 passages. Hepatic and Pancreatic cells were differentiated from the cells, also differentiated using serum free media. The cells were dedifferentiated under 0.1ng/10mL hydrogen sulfide. Hepatic differentiation was accelerated, since hepatic markers expressed more than in control, and as urea production was increased. But pancreatic was suppressed as insulin production was suppressed. The pulp stem cells themselves caused strong apoptosis under hydrogen sulfide exposure.

使用 CD117 抗体和磁力分离从人类乳牙和智齿中分离出干细胞。每 4 次传代，使用 CD117 抗体使用磁力分离对细胞进行传代。肝细胞和胰细胞从细胞中分化出来，也使用无血清培养基进行分化。细胞在0.1ng/10mL硫化氢下去分化。肝分化加速，因为肝标记物表达高于对照，且尿素产量增加。但随着胰岛素的产生受到抑制，胰腺也受到抑制。牙髓干细胞本身在硫化氢暴露下引起强烈的凋亡。

项目成果

Myrsinoic Acid B Inhibits the Production of Hydrogen Sulfide by Periodontal Pathogens

肉豆蔻酸 B 抑制牙周病原菌产生硫化氢

• 发表时间: 2010
• 期刊: J.Breath Res. 4
• 作者: Ito S;Shimura S;Tanaka T;Yaegaki K
• 通讯作者: Yaegaki K

Oral Malodorous Compound Induces Osteoclast Differentiation without RANKL

口腔恶臭化合物在没有 RANKL 的情况下诱导破骨细胞分化

• 发表时间: 2010
• 期刊: J Periodont 81
• 作者: Ii H;Imai T;Yaegaki K;Irie K;Ekuni D;Morita M
• 通讯作者: Morita M

Oral malodorous compound triggers mitochondrial-dependent apoptosis and causes genomic DNA damage in human gingival epithelial cells

• DOI: 10.1111/j.1600-0765.2008.01199.x
• 发表时间: 2010-02-01
• 期刊: JOURNAL OF PERIODONTAL RESEARCH
• 影响因子: 3.5
• 作者: Calenic, B.;Yaegaki, K.;Ii, H.
• 通讯作者: Ii, H.

Hydrogen sulfide induces apoptosis in epithelial cells derived from human gingiva

• DOI: 10.1088/1752-7155/2/1/017007
• 发表时间: 2008-03-01
• 期刊: JOURNAL OF BREATH RESEARCH
• 影响因子: 3.8
• 作者: Murata, T.;Yaegaki, K.;Ii, H.
• 通讯作者: Ii, H.

One shot of hydrogen sulfide application induces a transient osteoclast differentiation with RANKL expression in the rat model

注射一剂硫化氢可诱导大鼠模型中瞬时破骨细胞分化并表达 RANKL

• 发表时间: 2009
• 期刊: Archs Oral Biology 54
• 作者: Irie K;et al.
• 通讯作者: et al.