Study for the Process of Developing General Conditions Because of Periodontitis: Effects of Volatile Sulfur Compounds on Stem Cells

用CD 117抗体和磁性分离法从人乳牙和智齿中分离干细胞。使用磁性分离，使用CD 117抗体，每4次传代细胞。从细胞分化肝细胞和胰腺细胞，也使用无血清培养基分化。细胞在0.1ng/10 mL硫化氢中去分化。肝分化加速，因为肝标志物表达比对照组多，并且尿素产生增加。但是胰腺的胰岛素分泌受到抑制。牙髓干细胞本身在硫化氢暴露下引起强烈的凋亡。

Stem cells were isolated from human deciduous and wisdom teeth using CD117 antibody and magnetic separation. The cells were passaged using the magnetic separation using CD117 antibody at every 4 passages. Hepatic and Pancreatic cells were differentiated from the cells, also differentiated using serum free media. The cells were dedifferentiated under 0.1ng/10mL hydrogen sulfide. Hepatic differentiation was accelerated, since hepatic markers expressed more than in control, and as urea production was increased. But pancreatic was suppressed as insulin production was suppressed. The pulp stem cells themselves caused strong apoptosis under hydrogen sulfide exposure.