Study for the Process of Developing General Conditions Because of Periodontitis: Effects of Volatile Sulfur Compounds on Stem Cells

采用CD117抗体和磁分离技术从人乳牙和智齿中分离出干细胞。每隔4代用CD117抗体进行磁分离传代。从细胞中分化出肝细胞和胰腺细胞，并用无血清培养液进行分化。细胞在0.1 ng/10ml硫化氢溶液中去分化。肝脏分化加快，因为肝脏标志物的表达比对照多，并且尿素的产生增加。但由于胰岛素生成受到抑制，胰腺也受到了抑制。在硫化氢暴露下，牙髓干细胞本身会引起强烈的细胞凋亡。

Stem cells were isolated from human deciduous and wisdom teeth using CD117 antibody and magnetic separation. The cells were passaged using the magnetic separation using CD117 antibody at every 4 passages. Hepatic and Pancreatic cells were differentiated from the cells, also differentiated using serum free media. The cells were dedifferentiated under 0.1ng/10mL hydrogen sulfide. Hepatic differentiation was accelerated, since hepatic markers expressed more than in control, and as urea production was increased. But pancreatic was suppressed as insulin production was suppressed. The pulp stem cells themselves caused strong apoptosis under hydrogen sulfide exposure.