FEL & X-ray Single-particle 3D Imaging

通过该项目，我们建立了相干x射线衍射层析成像技术，常规成像生物细胞和细胞器的三维结构，分辨率在100nm以下。揭示了孢子酵母和哺乳动物细胞核的三维结构。在此基础上建立了FEL单次实验方案，实现了XFEL单粒子生物分子成像。进展包括小分子成像能力与微米尺寸聚焦同步加速器x射线和单粒子注入器的发展。

Through the project, we have established coherent x-ray diffraction tomography to image 3D structure of biological cells and organelles at sub 100nm resolution routinely. 3D structure of spore yeast and mammalian cellular nucleus were unveiled. Further we have established FEL single-shot experiment scheme to realize single-particle biomolecule imaging at XFEL. Progress includes the small molecule imaging capability with micron size focused synchrotron x-rays and also single particle injector development.