FEL & X-ray Single-particle 3D Imaging

自由电子实验室

• 批准号: 22681017
• 负责人: SONG Changyong
• 金额: $ 12.23万
• 依托单位: The Institute of Physical and Chemical Research
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (A)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22681017/
• 关键词: coherent x-rays; single molecules; single particle injector; x-ray focusing; 3D imaging; XFEL; single-shot imaging; coherent x-ray; bio-imaging

Through the project, we have established coherent x-ray diffraction tomography to image 3D structure of biological cells and organelles at sub 100nm resolution routinely. 3D structure of spore yeast and mammalian cellular nucleus were unveiled. Further we have established FEL single-shot experiment scheme to realize single-particle biomolecule imaging at XFEL. Progress includes the small molecule imaging capability with micron size focused synchrotron x-rays and also single particle injector development.

通过该项目，我们建立了相干x射线衍射层析成像技术，常规成像生物细胞和细胞器的三维结构，分辨率在100nm以下。揭示了孢子酵母和哺乳动物细胞核的三维结构。在此基础上建立了FEL单次实验方案，实现了XFEL单粒子生物分子成像。进展包括小分子成像能力与微米尺寸聚焦同步加速器x射线和单粒子注入器的发展。

项目成果

Nano-scale imaging with coherent diffraction microscopy : From synchrotron x-rays to free electron lasers

使用相干衍射显微镜进行纳米级成像：从同步加速器 X 射线到自由电子激光器

• 发表时间: 2011
• 作者: Shinya Kasai;YoshiChka Otani;Yasuhiro Fukuma;Kazuhito Tsukagoshi;Jun-ichiro Ohe;Sadamichi Maekawa;and Seiji Mitani;C. Song
• 通讯作者: C. Song

Diffraction Microscopy in Molecular Imaging

分子成像中的衍射显微镜

• 发表时间: 2011
• 作者: H.Fujiwara;T.Ikeda;K.Sasaki;青柳星見,松永行子,松井等,大久保有希,竹内昌治;C. Song
• 通讯作者: C. Song

Preparation of experiments-Coherent Diffraction Imaging

实验准备-相干衍射成像

• 发表时间: 2011
• 作者: 熊谷国憲;品田賢宏;他;Zhiqiang Guo;C. Song
• 通讯作者: C. Song

Coherent Diffraction Imaging and XFEL

相干衍射成像和 XFEL

• 发表时间: 2012
• 作者: 杉江恒二;芳村毅;C. Song
• 通讯作者: C. Song

Biological 3D imaging with Intense, Coherent X-rays

使用强相干 X 射线进行生物 3D 成像

• 发表时间: 2010
• 作者: C. Song