Functionalized conducting polymer biointerfaces for cell engineering: specific adhesion of proteins and cells, cell patterning, and dynamic binding/releasing by electrical stimuli.

在这个最初项目的3年时间里，我们成功地实现了所有最初的目标，并建立了一个新的概念，利用分子设计的导电聚合物来控制细胞在细胞和材料之间的界面上的行为。界面也是动态的，因此我们可以通过化学刺激和电刺激来控制界面。取得的主要成果包括：(1)成功构建了由20多个新型噻吩基单体组成的单体文库。(2)通过引入禁止蛋白质和细胞与材料结合的单体来控制细胞与材料的相互作用。(3)改变层状聚噻吩组装体的表面性质。(4)自上而下制造自下而上的电聚合组件的单元图案化。(5)电刺激下的聚噻吩池动态界面。我们用这些材料进一步研究了化学和电刺激对细胞工程的影响。主要结果包括：(1)用于细胞工程的导电聚合物纳米网络。通过控制电聚合反应动力学，含有多种官能团的噻吩类单体可以形成纤维尺寸在20~40 nm之间的EDOT纳米网络。(2)用捕捉剂强化纳米结构导电聚合物的细胞捕获。抗体偶联的聚噻吩纳米点对癌细胞的捕获效率更高。

During the 3-year of this original project, we have successfully achieved all the original goals and build a new concept to utilize molecularly designed conducting polymer to control cellular behavior on the interface between cells and materials. The interface is also dynamic therefore we can control the interface by both chemical and electrical stimulation. The major achievements include: (1) Successfully build a monomer library consists of more than 20 new thiophene based monomers. (2) Control the cellular interaction with the materials with introduction of the monomers that prohibit proteins and cells binding to the materials. (3) Switch the surface properties from layered polythiophenes assemblies. (4) Cell patterning with top-down fabrication of bottom-up electropolymerization assemblies. (5) Dynamic polythiophene-cell interface with electrical stimulation. We further studied the effect of chemical and electrical stimulation on the cell engineering with these materials. The major results include: (1) Conducting polymer nano-networks for cell engineering. Thiophene monomer with a variety of functional groups would form EDOT nano-networks with the nanofiber dimensions between 20 to 40 nm by controlling the electropolymerization kinetics. (2) Enhanced cell-capturing on the nanostructured conducting polymer with capture agents. More efficient capturing of cancer cells were demonstrated on antibody conjugated polythiophene nanodots.