Functional characterization of redox-sensitive ion channels usingchemical library

在哺乳动物中，感知细胞内氧化还原状态对我们的生命至关重要。最近，一类TRP通道已被证明可以作为各种环境变化的细胞传感器。在本研究中，我们构建了具有不同氧化还原性质的化学文库，以精确表征TRP通道的氧化还原敏感性。我们的表征发现TRPA1是一个高度敏感的氧化和还原通道。重要的是，TRPA1通道被分子氧激活，并对小鼠的氧感应机制起关键作用。

In mammals, it is essential for our life to sense intracellular redox status. Recently, a class of TRP channels has been demonstrated to act as cell sensors for various environmental changes. In this research, we constructed chemical library having various redox properties to precisely characterize the redox sensitivity of TRP channels. Our characterization identified that TRPA1 is a highly sensitive channel for oxidation and reduction. Importantly, TRPA1 channel is activated by molecular oxygen, and critically contributes to oxygen-sensing mechanisms in mice.