Visualization techniques for electrokinetic flow dynamics in confined nanospace

在这项研究中，我们开发了纳米空间电动流动动力学的测量技术，并将我们的技术应用于开发生物大分子分析的新技术。我们首先研究了生物大分子在纳米通道和纳米孔中的电泳流，发现纳米限制降低了生物大分子的迁移率。我们第二次将我们的测量技术扩展到研究具有微孔的电池电穿孔中的非稳定离子流动。最后，我们提出了一种集成芯片上电穿孔和等速电泳法从单细胞中同时提取、分离和定量RNA和DNA的技术。

In this study, we have developed measurement techniques for electrokinetic flow dynamics in nano-confiend spaces and applied our techniques to develop novel techniques for the analyses of biomacromolecules. We first investigated electrophoretic flows of biomacromolecules in nano channels and nano pores, and found the nano-confinement decreases the mobility of the biomacromolecules. We second extended our measurement technique to investigate unsteady ionic flows in the cell electroporation with micro-pores. We lastly proposed a technique for simultaneously extracting, separating, and quantifying RNA and DNA from single cells by integrating on-chip electroporation and isotachophoresis.