Systematic analyses of modes of migration, differentiation andfunction of bone-resorbing osteoclasts visualized by intravitalimaging

活体成像可视化破骨细胞迁移、分化和功能模式的系统分析

• 批准号: 22689030
• 负责人: ISHII Masaru
• 金额: $ 12.56万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (A)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22689030/
• 关键词: リウマチ学; 骨代謝; 破骨細胞; イメージング; 関節リウマチ; ケモカイン; 脂質メディエーター; 骨粗鬆症; 細胞動態

The main subject of this study is to reveal the cellular dynamics in various kinds of tissues and organs in vivo, by using advanced imaging techniques, especially focusing on the dynamic phenomenon within bone marrow cavity. By improving the optical technology, we developed an intravital bone imaging system that enabled elucidation of control mechanisms for dynamic behaviors of bone-resorbing osteoclasts in vivo. We also developed novel anti-bone resorptive treatments based on our findings.

本研究的主要目的是利用先进的成像技术，揭示体内各种组织和器官的细胞动力学，特别是骨髓腔内的动态现象。通过改进光学技术，我们开发了一种活体骨成像系统，该系统能够阐明体内骨吸收破骨细胞动态行为的控制机制。根据我们的发现，我们还开发了新的抗骨吸收治疗方法。

项目成果

Intestinal CX3C chemokine receptor 1high (CX3CR1high) myeloid cells prevent T-cell-dependent colitis

• DOI: 10.1073/pnas.1114931109
• 发表时间: 2012-03-27
• 期刊: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• 影响因子: 11.1
• 作者: Kayama, Hisako;Ueda, Yoshiyasu;Takeda, Kiyoshi
• 通讯作者: Takeda, Kiyoshi

Systemic Circulation and Bone Recruitment of Osteoclast Precursors Tracked by Using Fluorescent Imaging Techniques

• DOI: 10.4049/jimmunol.1201345
• 发表时间: 2013-01-15
• 期刊: JOURNAL OF IMMUNOLOGY
• 影响因子: 4.4
• 作者: Kotani, Manato;Kikuta, Junichi;Ishii, Masaru
• 通讯作者: Ishii, Masaru

Layer V cortical neurons require microglial support for survival during postnatal development

• DOI: 10.1038/nn.3358
• 发表时间: 2013-05-01
• 期刊: NATURE NEUROSCIENCE
• 影响因子: 25
• 作者: Ueno, Masaki;Fujita, Yuki;Yamashita, Toshihide
• 通讯作者: Yamashita, Toshihide

Dynamic analysis of histamine-mediated attenuation of acetylcholine-induced sweating via GSK3beta activation

通过 GSK3beta 激活组胺介导的乙酰胆碱诱导出汗减弱的动态分析

• 发表时间: 2013
• 期刊: J. Invest. Dermatol
• 作者: Matsui S;Murota H;Takahashi A;Yang A;Lee J-B;Omiya K;Ohmi M;Kikuta J;Ishii M;Katayama I
• 通讯作者: Katayama I

Intravital two-photon imaging: a versatile tool for dissecting the immune system

• DOI: 10.1136/ard.2010.138156
• 发表时间: 2011-03-01
• 期刊: ANNALS OF THE RHEUMATIC DISEASES
• 影响因子: 27.4
• 作者: Ishii, Taeko;Ishii, Masaru
• 通讯作者: Ishii, Masaru