Genetic analysis of the hindbrain neuronsthat determine the escape direction in zebrafish

决定斑马鱼逃逸方向的后脑神经元的遗传分析

• 批准号: 22700349
• 负责人: ASAKAWA Kazuhide
• 金额: $ 2.5万
• 依托单位: National Institute of Genetics
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22700349/
• 关键词: ロコモーション; 方向; 後脳; ゼブラフィッシュ; Gal4; mafb; TeTxLC; Mauthner; 逃避行動; GAL4; Cre; BAC; 脳幹; tatanus toxin; 逃避運動

An animal moving through the environment maintains or changes its direction of progression based on internal and/or external sensory information. Such directional control of locomotion must be precisely regulated in escape behaviors, which determine whether an animal can survive a predatory attack. Here, we studied the neural mechanisms underlying the directional control of escape locomotion against tactile stimulus in zebrafish. We found that, when the tetanus neurotoxin light chain was induced in a hindbrain domain that expressed the mafb-transcription factor gene, embryos displayed a lateral escape turn with a larger angle, thereby escaping in an abnormal direction. The increase in the lateral turn angle was observed when the touch was applied to the head, as well as tail region. These observations suggest that the mafb-positive domain in the hindbrain can discriminate the position of the tactile sensory stimulus and determine accordingly an angle of lateral turn to select a proper direction of escape locomotion

在环境中移动的动物基于内部和/或外部感觉信息保持或改变其前进方向。这种对运动方向的控制必须在逃跑行为中得到精确的调节，这决定了动物是否能在捕食性攻击中幸存下来。在这里，我们研究的神经机制背后的方向控制的逃避运动对触觉刺激的斑马鱼。我们发现，当破伤风神经毒素轻链被诱导在后脑域表达的mafb转录因子基因，胚胎表现出一个横向逃逸转向与一个更大的角度，从而逃避在一个异常的方向。当触摸被施加到头部以及尾部区域时，观察到横向转动角度的增加。这些结果表明，MAFB阳性结构域可以区分触觉刺激的位置，并据此决定侧转角度，以选择适当的逃避运动方向

项目成果

糖鎖結合タンパク質遺伝子//lman2la/VIPL//の変異は逃避行動のラテラリティーに異常を引き起こす

碳水化合物结合蛋白基因 //lman2la/VIPL// 突变导致逃避行为偏侧性异常

• 发表时间: 2010
• 作者: Rvuhei Okumura;Dapeng Cai;Shenglan Li;Xianxiang Xu;Jie Li;浅川和秀
• 通讯作者: 浅川和秀

Role of the L-type lectin VIPL/Lman2la in the development of escape locomotion in zebrafish

L型凝集素VIPL/Lman2la在斑马鱼逃逸运动发展中的作用

• 发表时间: 2012
• 作者: Asakawa;K.
• 通讯作者: K.

Migration of Neuronal Precursors From the Telencephalic Ventricular Zone Into the Olfactory Bulb in Adult Zebrafish

• DOI: 10.1002/cne.22722
• 发表时间: 2011-12-01
• 期刊: JOURNAL OF COMPARATIVE NEUROLOGY
• 影响因子: 2.5
• 作者: Kishimoto, Norihito;Alfaro-Cervello, Clara;Sawamoto, Kazunobu
• 通讯作者: Sawamoto, Kazunobu

Glycinergic transmission and postsynaptic activation of CaMKII are required for glycine receptor clustering in vivo

• DOI: 10.1111/gtc.12032
• 发表时间: 2013-03
• 期刊: Genes to Cells
• 影响因子: 2.1
• 作者: Iori Yamanaka;Mariko Miki;K. Asakawa;K. Kawakami;Y. Oda;Hiromi Hirata

GENETIC DISSECTION OF THE HINDBRAIN BY THE Gal4-UAS SYSTEM

Gal4-UAS 系统对后脑进行基因解剖

• 发表时间: 2011
• 作者: Asakawa;K.
• 通讯作者: K.