The functional analysis of a novel ubiquitin like posttranslational modifier UBL3

通过生物信息学方法，我们确定UBL3是一个高度保守的翻译后修饰因子。我们发现UBL3定位在质膜上，并与其他蛋白质结合，如泛素蛋白。然而，UBL3的功能仍不清楚。为了阐明UBL3的功能，我们寻找了与UBL3相互作用的蛋白，并鉴定了几种与UBL3结合的蛋白。一个UBL3结合蛋白促进了UBL3的翻译后修饰，UBL3是通过UBL3进行的翻译后修饰。这些发现表明，UBL3可能作为一个新的膜定位系统的重要组成部分发挥关键作用。

By using a bioinformatical method, we identified UBL3 as a highly conserved posttranslational modifier. We found that UBL3 were localized at the plasma membrane and attached to other proteins like an ubiquitin protein. However, the functional role of UBL3 is still unknown. To elucidate roles of UBL3, we searched for interaction protein with UBL3.As a result, we identified several UBL3-binding proteins. One UBL3-binding protein enhanced UBL3ylation which is a posttranslational modification via UBL3.These findings suggest that UBL3 may play a pivotal role as an essential component of a novel membrane localization system.