Analysis of the neuropathological mechanism by using calpain mutant mice

在本研究中，我们分析了calain-1和calain-2双基因敲除小鼠的运动异常。在Morris水迷宫实验中，神经元特异性缺失基因敲除显示运动和/或视觉能力异常。然后，我们通过光谱技术和iTRAQ方法鉴定了几种与功能异常有关的Calain-1和Calain-2底物。然而，一些底物并没有被钙蛋白酶如此多地切割。因此，我们正试图利用钙离子载体来鉴定细胞培养系统中的钙蛋白酶底物。

In this study, we have analyzed the motor abnoromality in calpain-1 and calpain-2 double knockout mice. Neuron-specific deleted knockout showed abnormalities in motor and/or visual capacity in Morris water maze test. Then, we identified several substrates of calpain-1 and calpain-2, which relate the functional abnormalities, by masspectrometic technique and the iTRAQ method. However, some of the substrates were not cleaved so much by calpain. Thus, we are trying to identify calpain substrates in cell culture system using calcium ionophores.