Functional dissection of retinal neurons and their dendrites by optogenetic approaches with ectopic melanopsin expression

通过异位黑视蛋白表达的光遗传学方法对视网膜神经元及其树突进行功能解剖

• 批准号: 22700421
• 负责人: KOIZUMI Amane
• 金额: $ 2.5万
• 依托单位: National Institute for Physiological Sciences
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22700421/
• 关键词: 脳・神経; 神経科学; 生理学; 脳神経疾患; バイオテクノロジー

The aim of this study is to investigate the function of retinal interneurons, namely amacrine cells, and their dendrites by optogenetic approaches with ectopic expression of photosensitive pigment, melanopsin. We successfully established organotypic tissue culture of adult rodent retina with particle-mediated gene transfer of melanopsin. In addition, we established transgenic mouse line, BitetO-OPN4-mCherry, which express melanopsin(OPN4) as well as mCherry under the presence of tetracycline-activator(tTA).

本研究旨在通过异位表达光敏色素黑素的光遗传学方法研究视网膜中间神经元，即无长突细胞及其树突的功能。我们成功地建立了颗粒介导的黑素基因转移成年啮齿动物视网膜的器官型组织培养。此外，我们还建立了转基因小鼠BitetO-OPN4-mCherry，在四环素激活剂(TTA)存在下表达黑素(OPN4)和mCherry。

项目成果

Asymmetric inhibitory connections enhance directional selectivity in a three-layer simulation model of retinal networks.

不对称抑制连接增强了视网膜网络三层模拟模型中的方向选择性。

• DOI: 10.1142/s0219635210002469
• 发表时间: 2010
• 期刊: Journal of integrative neuroscience
• 影响因子: 1.8
• 作者: A. Koizumi;M. Takayasu;H. Takayasu
• 通讯作者: H. Takayasu

Spatial Regularity in the Arrangement of Excitatory Synapses Within the Inner Plexiform Layer. Manipulating gene expression in organotypic tissue culture of adult rat retina

内丛状层内兴奋性突触排列的空间规律性。

• 发表时间: 2010
• 作者: Takahashi Y.;Tsuji O.;Kumagai G.;Hara M. C.;Okano J. H.;Miyawaki A.;Toyama Y.;Okano H.;Nakamura M.;AMANE KOIZUMI
• 通讯作者: AMANE KOIZUMI

MANIPULATING GENE EXPRESSION IN ORGANOTYPIC TISSUE CULTURE OF ADULT RODENT RETINA

操纵成年啮齿动物视网膜器官组织培养中的基因表达

• 发表时间: 2011
• 作者: Satoru Moritoh;Kenji F Tanaka;Hiroshi Jouhou;Kazuyuki Ikenaka;Amane Koizumi
• 通讯作者: Amane Koizumi

Endogenous arginine vasopressin–positive retinal cells in arginine vasopressin–eGFP transgenic rats identified by immunohistochemistry and reverse transcriptase–polymerase chain reaction

通过免疫组织化学和逆转录聚合酶链反应鉴定精氨酸加压素-eGFP转基因大鼠的内源性精氨酸加压素阳性视网膜细胞

• 发表时间: 2011
• 期刊: Molecular Vision
• 影响因子: 2.2
• 作者: Satoru Moritoh;Kaori Sato;Y. Okada;A. Koizumi
• 通讯作者: A. Koizumi

Manipulation of neuronal activity by ectopic expression of melanopsin

通过黑视蛋白的异位表达来操纵神经元活动

• 发表时间: 2011
• 作者: Kenji F Tanaka;Akihiro Yamanka;Amane Koizumi
• 通讯作者: Amane Koizumi