Quantitative analysis of molecular dynamics in the transduction cascade using caged compounds and imaging techniques

嗅觉传导级联始于与气味分子结合的受体蛋白。结合后，G蛋白和AC被持续激活。在[cAMP]i和[Ca+]i升高后，它导致开放转导通道(CNG，Cl(Ca))并产生电流反应。胞浆元素(cAMP、Ca~(2+))的时空分布是影响嗅觉转导级联反应的关键因素，本文从嗅觉适应和非线性信号放大两个方面对其进行了研究。1)局部激光刺激(LMS-ROI)。2)笼式cAMP光解(膜片钳)和3)钙成像(Fluo4)并行的电流响应。

Olfactory transduction cascade starts from the receptor protein which binds to odorant molecules. After the binding, G-protein and AC are activated continuously. It leads to open the transduction channels (CNG, Cl(Ca)) and generate current responses, after [cAMP]i and [Ca2+]i increased. Spatial and temporal distributions of cytoplasmic elements (cAMP, Ca2+) are key factors in the olfactory transduction cascade.We examined that based on the olfactory adaptation and non-linear signal amplificationwith these techniques. 1) Local laser light stimulation (LMS-ROI). 2) Current responses with caged-cAMP photolysis (patch-clamp) and 3) Ca-imaging(Fluo4) in parallel.