Establishment of producing method for detection antibody for primate using DNA immunization technology

松鼠猴（Saimiri sciureus）是研究疟疾疫苗开发等传染病的宝贵实验动物；然而，这种动物的IgG分子尚未被表征。抗体依赖性细胞毒性是一种抗体介导的免疫反应，对排除病原体或癌细胞很重要。在人类中，IgG1或IgG3与Fc受体Fcγ riiia相互作用。先前的研究表明，人fc - γ riiia在残基176处含有一个取代（Phe→Val），除了与IgG1和IgG3相互作用外，还可以与IgG4相互作用。在这里我们发现松鼠猴IgG与它的FcγRIIIa相互作用。最后，制备了高敏感性和特异性的抗松鼠猴IgG单克隆抗体，用于western blotting和ELISA检测。

The squirrel monkey (Saimiri sciureus) is a valuable experimental animal for research into infectious diseases such as malaria vaccine development; however, the IgG molecules in this animal are not yet to be characterized. Antibody-dependent cellular cytotoxity is an antibody-mediated immune response and is important for exclusion of pathogens or cancer cells. In human, IgG1 or IgG3 interacts with the Fc receptor, FcγRIIIa. Previous studies have revealed that human FcγRIIIa contains a substitution (Phe→Val) at residue 176 that can interact with IgG4 in addition to IgG1 and IgG3. Here we show that the squirrel monkey IgG interacts with its FcγRIIIa. Finally, highly sensitive and specific anti-squirrel monkey IgG monoclonal antibodies were developed for used in western blotting and ELISA assays.