Establishment of producing method for detection antibody for primate using DNA immunization technology

松鼠猴（Saimiri sciureus）是研究传染病如疟疾疫苗开发的有价值的实验动物;然而，该动物中的IgG分子尚未被表征。抗体依赖性细胞毒性是抗体介导的免疫应答，对于排除病原体或癌细胞很重要。在人体中，IgG 1或IgG 3与Fc受体FcγRIIIa相互作用。先前的研究表明，人FcγRIIIa在残基176处含有一个取代（Phe→瓦尔），除IgG 1和IgG 3外，该取代还可与IgG 4相互作用。在这里，我们表明松鼠猴IgG与其FcγRIIIa相互作用。最后，高灵敏度和特异性的抗松鼠猴IgG单克隆抗体的开发用于蛋白质印迹和ELISA测定。

The squirrel monkey (Saimiri sciureus) is a valuable experimental animal for research into infectious diseases such as malaria vaccine development; however, the IgG molecules in this animal are not yet to be characterized. Antibody-dependent cellular cytotoxity is an antibody-mediated immune response and is important for exclusion of pathogens or cancer cells. In human, IgG1 or IgG3 interacts with the Fc receptor, FcγRIIIa. Previous studies have revealed that human FcγRIIIa contains a substitution (Phe→Val) at residue 176 that can interact with IgG4 in addition to IgG1 and IgG3. Here we show that the squirrel monkey IgG interacts with its FcγRIIIa. Finally, highly sensitive and specific anti-squirrel monkey IgG monoclonal antibodies were developed for used in western blotting and ELISA assays.