Establishment of producing method for detection antibody for primate using DNA immunization technology

松鼠猴(Saimiri Scureus)是研究疟疾疫苗等传染病的有价值的实验动物；然而，这种动物中的免疫球蛋白分子还没有被表征。抗体依赖的细胞毒性是一种抗体介导的免疫反应，对于排除病原体或癌细胞很重要。在人类中，免疫球蛋白G_1或免疫球蛋白G_3与Fc受体FcγRIIa相互作用。以前的研究表明，人FcγRIIIa在第176位含有一个替代(Phe→Val)，除了与Ig G1和Ig G3相互作用外，还可以与Ig G4相互作用。在这里，我们显示了松鼠猴免疫球蛋白与其FcγRIIIa相互作用。最后，研制出高灵敏度、高特异性的抗松鼠猴Ig G单抗，用于免疫印迹和ELISA检测。

The squirrel monkey (Saimiri sciureus) is a valuable experimental animal for research into infectious diseases such as malaria vaccine development; however, the IgG molecules in this animal are not yet to be characterized. Antibody-dependent cellular cytotoxity is an antibody-mediated immune response and is important for exclusion of pathogens or cancer cells. In human, IgG1 or IgG3 interacts with the Fc receptor, FcγRIIIa. Previous studies have revealed that human FcγRIIIa contains a substitution (Phe→Val) at residue 176 that can interact with IgG4 in addition to IgG1 and IgG3. Here we show that the squirrel monkey IgG interacts with its FcγRIIIa. Finally, highly sensitive and specific anti-squirrel monkey IgG monoclonal antibodies were developed for used in western blotting and ELISA assays.