Analysis of the stress protein in primates

灵长类动物应激蛋白的分析

基本信息

批准号：
22700460
负责人：
HIGASHINO Atsunori
金额：
$ 1.58万
依托单位：
Kyoto University (2011)独立行政法人医薬基盤研究所 (2010)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Young Scientists (B)
财政年份：
2010
资助国家：
日本
起止时间：
2010 至 2011
项目状态：
已结题

项目摘要

Calreticulin(Crt) is a molecular chaperone ubiquitously present in the endoplasmic reticulum. In non-human primates, age-related occurrence of anti-Crt antibody has not been reported. We developed an ELISA assay for an anti-Crt antibody and determined the age-related increase in the levels of anti-Crt antibody in three groups of cynomolgus monkeys : juvenile(1. 5 yr), young adults(5. 10yr) and aged adults(20. 34 yr). Mean±SD auto-antibody levels at 450 nm in juvenile, young adults and aged groups were 0. 23±0. 18, 0. 30±0. 28, and 0. 55±0. 33, respectively. Statistically significant differences were noted in the autoantibody levels to Crt among the aged group and juvenile or young adults. This is the first report to demonstrate the expression of anti-Crt autoantibody in aged monkeys and indicates that cynomologous monkeys may serve as an appropriate nonhuman primate model for studies of age-related alteration of immune function in elderly humans.Next, we examined the effects of exogenous Crt for gene expression in macaque blood cells. Transcriptome data from whole blood cells of cynomolgus monkeys were obtained using the next-generation sequencer SOLiD. The short-read tags obtained from this analysis were mapped on the rhesus macaque genome sequence. Addition of exogenous Crt protein increased some gene expressions, such as cytoskeleton remodeling, immune, and cell adhesion. The data show a possibility of application of stress proteins for veterinary medical research of macaque monkeys.

钙网蛋白（CRT）是内质网中普遍存在的分子链酮。在非人类素数中，尚未报道与年龄有关的抗CRT抗体的发生。我们开发了一种用于抗CRT抗体的ELISA测定法，并确定了三组cynomolgus猴子的抗CRT抗体水平的增加：少年（1.5岁），年轻人（5.10年）和老年人（20.34岁）。少年，年轻人和老年人组的平均±SD自动抗体水平为450 nm，为0。23±0。 18，0。30±0。 28和0。55±0。分别为33。在年龄组和少年或年轻人中，自身抗体水平的自身抗体水平上有统计学上的显着差异。这是第一个报道，证明了老年猴子抗CRT自身抗体的表达，并表明cynomeloguologusion猴子可以用作适当的非人类私人模型，用于研究基本人体中与年龄相关的免疫功能变化的研究，我们检查了乳酸血细胞中外源性CRT对基因表达的影响。使用下一代序列固体获得了来自Cynomolgus猴子全血细胞的转录组数据。从该分析中获得的简读标签映射在恒河猕猴的基因组序列上。外源性CRT蛋白的添加增加了一些基因表达，例如细胞骨架重塑，免疫和细胞粘附。数据表明，将应力蛋白应用于猕猴的兽医医学研究可能性。