Proteomic analysis of nitrated proteins that change with aging and physical exercise in the brain

我们在成年大鼠海马中鉴定出12种含6-硝基色氨酸的蛋白，包括几种细胞骨架蛋白和糖酵解酶，并通过蛋白质组学分析确定了几种蛋白中具有显著离子评分水平（p<0.05）的硝化色氨酸残基的位置。6-硝基色氨酸蛋白含量随年龄增长呈增加趋势。另一方面，体育锻炼对12月龄大鼠抗6-硝基色氨酸抗体阳性斑点的影响不明显。

We identified twelve 6-nitrotyptophan-containing proteins including several cytoskeletal proteins and glycolytic enzymes in the adult rat hippocampus and determined the position of nitrated tryptophan residue with significant ion score levels(p<0.05) in several proteins by proteomic analysis. In addition, the amount of 6-nitrotyptophan-containing protein tended to increase with age. On the other hand, physical exercise on the 12 months old rats did not induce much difference in the positive spots of anti-6-nitrotryptophan antibody.