Proteomic analysis of nitrated proteins in the skeletal muscle after physical exercise.

我们从生理条件下的成年大鼠骨骼肌中鉴定出含硝基色氨酸的蛋白。高强度间歇跑步对骨骼肌中色氨酸的硝化程度没有影响。因此，含硝基色氨酸的蛋白质在生理条件下可能不能作为活性氧和活性氮的清除剂。此外，高强度间歇跑步没有改变α -骨骼肌肌动蛋白的色氨酸硝化作用（约60 kDa）和参与细胞信号转导的ERK。

We identified nitrotyptophan-containing proteins from adult rat skeletal muscle under physiological condition. The degree of typtophan nitration in the skeletal muscle were not changed by high intensity intermittent running. Thus, nitrotyptophan-containing proteins under physiological condition may not function as a scavenger of reactive oxygen and nitrogen species. In addition, the typtophan nitration of alpha-skeletal muscle actin, which is detected at approximately 60 kDa, and ERK that is involved in the signal transduction in cells were not changed by high intensity intermittent running.