Development of novel controlling of protozoa without anti-drugs for serving of safety chicken meat

鸡球虫病是由艾美耳球虫原生动物寄生虫引起的。以腹泻为主要特征。这些寄生虫给世界家禽业造成了重大的经济损失。我们对柔嫩艾美耳球虫的胞外产孢期和肠道发育阶段进行了全面的基因表达分析，以了解它们对感染的生物学作用。利用下一代测序仪对基因进行测序，发现组装的重叠群含有柔嫩艾美耳球虫的新基因。利用基因芯片技术对感染鸡盲肠第二代裂殖体增殖分化过程中上调或下调的基因进行了鉴定。这些结果表明，已鉴定的基因可能是控制该病的新靶点，如疫苗抗原。

Chicken coccidiosis is caused by protozoan parasites of Eimeria spp. and mainly characterized as diarrhea. These parasites are responsible for significant economic losses to the worldwide poultry industry. We performed comprehensive gene expression analyses on extracellular sporulation and intestinal developmental stages of E. tenella to understand their biology for the infection. By sequencing of cDNA using the next generation sequencer, assembled contigs were found to contain novel genes of E. tenella. The up- or down- regulated genes at proliferation and differentiation of the second generation schizonts in infected chicken ceca were identified by microarray analysis. These results suggested that the identified genes could be novel targets such as vaccine antigens to control the disease.