Identification of new target proteins for metastasis suppressor protein Nm23-H1

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Nm23-H1 is known as the suppressor of tumor metastasis. To find the potential target of Nm23-H1 in epithelial cell, we used matrix assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry (MS) analysis and N-terminal sequence analysis. We obtained 73 candidate proteins as a cellular target of Nm23-H1 including previous reported molecules. Those are categorized 4 groups which are involved in 8 cell structure proteins, 4 cell adhesion molecules, 20 signal molecules, and 12 cell constitution protein/unknown function molecules. One of candidatesspermatogenesis associated 13 (SPATA13) that is also known as adenomatous polyposis coli (APC)-stimulated guanine nucleotide exchange factor 2 (Asef2) observed in several tumors and associated with a decrease of cell to cell adhesion.